Stomatal morphology and physiology explain varied sensitivity to abscisic acid across vascular plant lineages
Stomatal morphology and physiology explain varied sensitivity to abscisic acid across vascular plant lineages
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DOI：
10.1093/plphys/kiab090
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发表时间：
2021-02-23
期刊：
影响因子：
7.4
通讯作者：
Fang, Xiang-Wen
中科院分区：
文献类型：
--
作者：
Gong, Lei;Liu, Xu-Dong;Fang, Xiang-Wen
脱落酸（阿坝）能诱导种子植物气孔迅速关闭，但对蕨类植物和石松植物气孔的作用尚不清楚。在这里，ABA诱导的气孔关闭，信号成分，保卫细胞K+和Ca 2+通量，液泡和肌动蛋白细胞骨架的动态，和保卫细胞原生质体的渗透系数（P-F）进行了分析，物种跨越的多样性维管陆地植物，包括11种种子植物，6蕨类植物，1石松。我们发现，所有11种种子植物表现出ABA诱导气孔关闭，但蕨类植物和石松物种没有。ABA诱导的过氧化氢升高，观察到在所有物种中，但下游的一氧化氮的生产，包括离子通道激活的信号转导途径，只观察到在种子植物。在被子植物蚕豆（蚕豆），阿坝应用引起大液泡室解体，肌动蛋白丝解体成短片段和P-f增加。没有观察到这些变化的蕨类植物Matteuccia struthiopteris和石松卷柏与阿坝处理的保卫细胞，但高渗溶液诱导蕨类植物和石松气孔关闭。我们的研究结果表明，蕨类植物和石松植物和种子植物之间的气孔调节有很大的差异。重要的是，这些发现揭示了可能负责最早的种子植物中阿坝的气孔反应的进化的生理和生物物理机制。
Abscisic acid (ABA) can induce rapid stomatal closure in seed plants, but the action of this hormone on the stomata of fern and lycophyte species remains equivocal. Here, ABA-induced stomatal closure, signaling components, guard cell K+ and Ca2+ fluxes, vacuolar and actin cytoskeleton dynamics, and the permeability coefficient of guard cell protoplasts (P-f) were analyzed in species spanning the diversity of vascular land plants including 11 seed plants, 6 ferns, and 1 lycophyte. We found that all 11 seed plants exhibited ABA-induced stomatal closure, but the fern and lycophyte species did not. ABA-induced hydrogen peroxide elevation was observed in all species, but the signaling pathway downstream of nitric oxide production, including ion channel activation, was only observed in seed plants. In the angiosperm faba bean (Vicia faba), ABA application caused large vacuolar compartments to disaggregate, actin filaments to disintegrate into short fragments and P-f to increase. None of these changes was observed in the guard cells of the fern Matteuccia struthiopteris and lycophyte Selaginella moellendorffii treated with ABA, but a hypertonic osmotic solution did induce stomatal closure in fern and the lycophyte. Our results suggest that there is a major difference in the regulation of stomata between the fern and lycophyte plants and the seed plants. Importantly, these findings have uncovered the physiological and biophysical mechanisms that may have been responsible for the evolution of a stomatal response to ABA in the earliest seed plants.