INVERTEBRATE TISSUE-CULTURE AS A TOOL TO STUDY INSECT TRANSMISSION OF PLANT-VIRUSES
INVERTEBRATE TISSUE-CULTURE AS A TOOL TO STUDY INSECT TRANSMISSION OF PLANT-VIRUSES
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DOI：
10.1007/bf02633956
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发表时间：
1993-04-01
影响因子：
2.1
通讯作者：
CREAMER, R
中科院分区：
文献类型：
--
作者：
CREAMER, R
无脊椎动物组织培养是昆虫传播的植物病毒研究中未充分利用的资源。自20多年前建立叶蝉载体单层以来，这些连续培养物主要用于测定在其昆虫载体中复制的植物病毒的感染性，并阐明伤口肿瘤植物呼肠孤病毒和马铃薯黄矮弹状病毒传播的病毒决定因素。用原代蚜虫培养物研究病毒的吸收和病毒积累。未来昆虫细胞培养的最大潜力是研究繁殖病毒的分子生物学。虽然叶蝉细胞系不支持在体内发现的相同病毒载体特异性，但细胞系在病毒转录物和突变病毒可以均匀地引入细胞中的容易性方面比使用整个昆虫具有一些优势。现有的叶蝉单层系统可以促进研究载体的遗传背景对病毒复制的影响。来源于唾液腺的培养物可能有助于研究识别病毒衣壳的昆虫受体。从稻飞虱，蓟马，和Eriophid螨载体的细胞系将允许研究的生物学和分子特征的植物病毒。
Invertebrate tissue culture is an underutilized resource in the study of insect-transmitted plant viruses. Since the establishment of leafhopper vector monolayers over 20 yr ago, these continuous cultures were used primarily to assay the infectivity of plant viruses that replicate in their insect vectors and to elucidate the viral determinants for transmission of wound tumor phytoreovirus and potato yellow dwarf rhabdovirus. Primary aphid cultures were used to study the uptake of viruses and viral accumulation. The biggest potential for use of insect cell culture in the future is in studying the molecular biology of propagative viruses. Although leafhopper cell lines do not support the same virus-vector specificity that is found in vivo, the cell lines have some advantage over the use of whole insects in the ease with which viral transcripts and mutant viruses can be uniformly introduced into cells. Existing leafhopper monolayer systems could facilitate studies on the influence of the vector's genetic background on viral replication. Cultures derived from salivary glands might be useful to study the insect receptors that recognize the viral capsid. Cell lines from planthoppers, thrips, and eriophid mite vectors would allow research on the biological and molecular characterization of poorly characterized plant viruses.