Photoperiodic regulation of the phospholipid molecular species composition in thoracic muscles and fat body of Pyrrhocoris apterus (Heteroptera) via an endocrine gland, corpus allatum
Photoperiodic regulation of the phospholipid molecular species composition in thoracic muscles and fat body of Pyrrhocoris apterus (Heteroptera) via an endocrine gland, corpus allatum
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DOI：
10.1016/s0022-1910(02)00188-9
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Zahradnícková, H
中科院分区：
文献类型：
--
作者：
Hodkova, M;Berková, P;Zahradnícková, H
传统观点认为，膜脂的冬季适应是由温度下降引起的。我们认为，Pyrrocoris apterus 的冬季膜重塑是由短日光周期引发的，然后温度下降和寒冷引起的变化代表了适应的后期阶段。短日光周期诱导滞育导致磷脂酰乙醇胺 (PE) 分子种类的积累，其中 C-16:0/C-18:2 酰基链被酯化到甘油的 sn-1/sn-2 位置，但牺牲了 C-18:0/C-18:2。与长日照昆虫相比，短日照昆虫胸肌（TM，15.0 vs. 8.2%）和脂肪体（1713，24.9 vs. 13.6%）中 C-16:0/C-18:2-PE 的比例有所增加。 C-16:0/C-18:2-PE 的比例在冷驯化过程中进一步增强（TM 中为 26.5%，FB 中为 33.6%），但代价是更饱和的物种 C-18:0/C-18:1-PE。这些变化在磷脂酰胆碱 (PC) 中不太明显。光周期的影响似乎是通过软体介导的。被剥夺了幼体的长日照非滞育雌性具有与短日照滞育雌性相似的磷脂分子种类特征。虽然酰基链重塑受光周期和温度调节，但头基组成仅受温度调节。与大多数其他生物体类似，与温暖温度（大于或等于 1 摄氏度）相比，低温（小于或等于 1 摄氏度）下 PE 水平较高（TM 为 50.3 比 43.5%，FB 为 44.3 比 37.8%），PC 水平较低（TM 为 35.9 比 40.2%，FB 为 41.6 比 46.1%）至 16 摄氏度）。与一般规则相反，PE 的不饱和度低于 PC。在 TM 和 FB 中，PC 中不饱和/不饱和分子种类的比例始终较高（TM 中为 56.3-67.5%，FB 中为 59.2-66.6%），而 PE 中的不饱和/不饱和分子种类比例始终较低（TM 中为 19.1-26.7%，FB 中为 12.1-15.1%）。结合已知的磷脂物理性质讨论了磷脂组成变化对低温和/或脱水应激的适应性意义。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
In the conventional view, the winter adaptation of membrane lipids is induced by temperature decrease. We propose that winter remodelling of membranes in Pyrrhocoris apterus is triggered by short-day photoperiod before the temperature decrease and changes caused by cold temperature represent the later phase of adaptation. The induction of diapause by short-day photoperiod results in an accumulation of phosphatidylethanolamine (PE) molecular species with C-16:0/C-18:2 acyl chains esterified to sn-1/sn-2 positions of glycerol at the expense of C-18:0/C-18:2. Proportions of C-16:0/C-18:2-PE are enhanced in short-day compared to long-day insects in both thoracic muscles (TM, 15.0 vs. 8.2%) and fat bodies (1713, 24.9 vs. 13.6%). Proportions of C-16:0/C-18:2-PE are further enhanced during cold acclimation (to 26.5% in TM, 33.6% in FB) at the expense of a more saturated species, C-18:0/C-18:1-PE. These changes are less prominent in phosphatidylcholines (PC). The effect of photoperiod seems to be mediated via the corpus allatum. Long-day non-diapause females deprived of their corpus allatum have the phospholipid molecular species profile similar to that found in short-day diapausing females. While the acyl chain remodelling is regulated by both photoperiod and temperature, the head group composition is regulated by temperature only. Similar to most other organisms, the level of PE is higher (50.3 vs. 43.5% in TM, 44.3 vs. 37.8% in FB) and that of PC is lower (35.9 vs. 40.2% in TM, 41.6 vs. 46.1% in FB) at cold temperatures (less than or equal to1degreesC) compared to warm temperatures (greater than or equal to16degreesC). In contrast to a general rule, the PE is less unsaturated than PC. In both TM and FB, proportions of unsaturated/unsaturated molecular species are consistently high in PC (56.3-67.5% in TM, 59.2-66.6% in FB), while they are consistently low in PE (19.1-26.7% in TM, 12.1-15.1% in FB). An adaptive significance of changes in the phospholipid composition for the low temperature and/or dehydration stress is discussed in relation to known physical properties of phospholipids. (C) 2002 Elsevier Science Ltd. All rights reserved.