Lubricin expression in the lumbar endplate and its association with Modic changes
Lubricin expression in the lumbar endplate and its association with Modic changes
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腰椎终板润滑素的表达及其与Modic变化的关系
DOI：
10.1016/j.jot.2019.10.009
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发表时间：
2020-05-01
影响因子：
6.6
通讯作者：
Zhao, Fengdong
中科院分区：
文献类型：
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作者：
Liu, Junhui;Wei, Xiaoan;Zhao, Fengdong
目的：探讨润滑素在腰椎终板中的表达及其与MODIC改变的关系。方法：取胸腰椎骨折或腰椎椎体间融合术患者的终板标本，分为两组：MCS组和正常组。免疫组织化学方法检测润滑素的表达，定量聚合酶链式反应(QPCR)分析各组之间的差异。使用兔模型证实了MCs终板和正常终板之间的润滑素表达和差异。在最后的实验中，将兔终板软骨细胞与痤疮丙酸杆菌(P.acnes)上清液共培养，检测润滑素和终板退变相关基因的表达。此外，在润滑剂过表达后，检测基质金属蛋白酶1(MMP1)、去整合素样和带有凝血酶反应蛋白5基序的金属蛋白酶(ADAMTS5)以及炎症因子(白介素1ss和白介素6)的表达。结果：人腰椎终板有润滑素表达，MCS组表达低于正常组。在兔模型中，终板中也发现了润滑剂。注射痤疮假单胞菌的兔(MCs组)终板润滑素表达较正常组降低。加入痤疮假单胞菌培养上清液后，兔终板软骨细胞的润滑素、聚集素、Sox9和II型胶原的表达显著降低，而基质金属蛋白酶-1和ADAMTS5的表达显著增加。此外，与阴性对照组相比，润滑素过表达可下调MMP1、ADAMTS5和炎症因子(IL-1ss和IL-6)的表达。结论：润滑剂存在于腰椎终板中，可能具有抗炎作用。痤疮假单胞菌感染可抑制软骨终板细胞表达润滑素，这可能促进软骨终板细胞的进展和终板退变。本文的翻译潜力：润滑剂可能具有抗炎作用。痤疮假单胞菌感染可抑制软骨终板细胞表达润滑素，这可能促进软骨终板细胞的进展和终板退变。
Objective: To explore the expression of lubricin in the lumbar endplate and its association with Modic changes (MCs). Methods: Human endplate specimens harvested from patients undergoing surgery for thoracolumbar spine fractures or lumbar interbody fusion were divided into two groups: MCs group and normal group. Lubricin expression was examined by immunohistochemistry, and differences between the groups were analysed using quantitative polymerase chain reaction (qPCR). Lubricin expression and differences between endplates with MCs and normal endplates were confirmed using a rabbit model. In a final experiment, rabbit endplate chondrocytes were cocultured with Propionibacteria acnes (P. acnes) supernatant, and the expression of lubricin and endplate degeneration related genes were evaluated. In addition, the expression of matrix metalloproteinase 1(MMP-1), A disintegrin-like and metalloproteinase with thrombospondin type 5 motif (ADAMTS5) and inflammatory factors (Interleukin- 1 ss (IL-1 ss) and Interleukin-6 (IL-6)) were evaluated after lubricin overexpression. Results: Lubricin was found in human lumbar endplates and its expression was lower in the MCs group compared to the normal group. In the rabbit model, lubricin was also found in the endplate. In rabbits injected with P. acnes (the MCs group), lubricin expression of endplate decreased compared to the normal group. In the culture of rabbit endplate chondrocytes with P. acnes supernatant, the expression of lubricin, aggrecan, sox9 and collagen type-II decreased significantly, while that of MMP-1 and ADAMTS5 increased significantly. Moreover, lubricin overexpression could downregulate the expression of MMP-1, ADAMTS5 and inflammatory factors (IL-1 ss and IL-6) compared to negative control. Conclusion: Lubricin is present in the lumbar endplate where it may have an anti-inflammatory role. P. acnes infection inhibits lubricin expression by cartilage endplate cells and this may facilitate the progression of MCs and endplate degeneration. The translational potential of this article: Lubricin may have an anti-inflammatory role. P. acnes infection inhibits lubricin expression by cartilage endplate cells and this may facilitate the progression of MCs and endplate degeneration.