Effects of antibiotic on microflora in ileum and cecum for broilers by 16S rRNA sequence analysis
Effects of antibiotic on microflora in ileum and cecum for broilers by 16S rRNA sequence analysis
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DOI：
10.1111/asj.13113
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发表时间：
2018-12-01
影响因子：
2
通讯作者：
Liu, Guohua
中科院分区：
文献类型：
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作者：
She, Yue;Cai, Huiyi;Liu, Guohua
本试验对肉鸡饲粮中添加和不添加抗生素时微生物的组成、丰度、动态分布和功能进行了分析和比较。采用16 SrRNA序列分析方法，对不同处理组雄性肉鸡肠道细菌组成进行了研究。选用1日龄AA肉仔鸡240只，随机分为2组，每组120只。治疗组在饲料中添加抗生素，而对照组不添加抗生素（对照组）。每个处理共评估10个重复。对照组饲喂含玉米、豆粕和棉籽粕的基础日粮，并满足营养需要。抗生素组在基础日粮基础上添加100 mg/kg金霉素。审判持续了42天。对所有给药组进行了操作分类单元划分和分类、α多样性、分类组成、β多样性和微生物区系比较分析沿着关键种属筛选。我们的数据表明，金霉素治疗肉鸡是直接相关的肠道内容物的特定细菌类群的变化，并在此提供见解抗生素对肉鸡盲肠和回肠微生物群落的影响。
An experiment was conducted to analyze and compare the microbial composition, abundance, dynamic distribution, and functions without and with antibiotic fed to broilers. A 16S rRNA-sequencing approach was used to evaluate the bacterial composition of the gut of male broilers under different groups. A total of 240 1-day old AA male broilers were randomly assigned to two groups, with 120 broilers per group. The treatment group was administered an antibiotic with their feed, while the control group was not administered antibiotic (control group). A total of 10 replicates were assessed per treatment. The control group was fed a basal diet containing corn, soybean meal, and cottonseed meal and met the nutritional requirement. The antibiotic group was fed 100 mg/kg aureomycin (based on the basal diet). The trial lasted 42 days. Operational taxonomic unit partition and classification, alpha diversity, taxonomic composition, beta diversity, and microflora comparative analyses along with key species screening were performed for all of the treatment groups. Our data indicate that aureomycin treatment in broilers is directly correlated with variations of the gut content of specific bacterial taxa, and herein provide insights into the impact of antibiotic on microbial communities in cecum and ileum of broiler chickens.