Protection from infection with Haemophilus influenzae type b by monoclonal antibody to the capsule.
Protection from infection with Haemophilus influenzae type b by monoclonal antibody to the capsule.
复制标题
通过胶囊的单克隆抗体防止 b 型流感嗜血杆菌感染。
DOI：
10.1093/infdis/146.2.249
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Insel,RA
中科院分区：
文献类型：
--
作者：
Gigliotti,F;Insel,RA
通过将免疫的 C57BL/6 小鼠的脾淋巴细胞与小鼠骨髓瘤系 P3-X63-Ag8.653 融合来产生 b 型流感嗜血杆菌的单克隆抗体。通过放射性标记抗原结合测定确定，一种杂交瘤 (5MIH9) 产生的抗体与荚膜多糖结合。该抗体属于 IgM 类，在体外与补体一起具有杀菌作用。在H幼鼠模型中检查了抗体5MIH9的保护和治疗能力。 b 型流感疾病。抗体（0.1 ml），未稀释或按 1:2、1:10 或 1:100 稀释，在腹腔注射 104–105H 前 4 小时施用。 b型流感病毒分别保护了100%、90%、55%和10%的动物。为了确定抗体5MIH9在治疗既定感染中的功效，在腹膜内注射细菌后每隔24小时给予抗体。延迟施用抗体5MIH9可有效降低菌血症的水平和发生率。
Monoclonal antibodies toHaemophilus influenzaetype b were produced by fusing splenic lymphocytes from immunized C57BL/6 mice with the mouse myeloma line P3-X63-Ag8.653. The antibody produced by one hybridoma (5MIH9) bound the capsular polysaccharide, as determined by a radiolabeled antigen-binding assay. The antibody was of the IgM class and was bactericidal in vitro with complement. The protective and therapeutic capacity of antibody 5MIH9 was examined in the infant rat model ofH. influenzaetype b disease. Antibody (0.1 ml), either undiluted or diluted 1:2, 1:10, or 1:100, administered 4 hr before intraperitoneal injection of 104–105H. influenzaetype b organisms protected 100%, 90%, 55%, and 10% of the animals, respectively. To determine the efficacy of antibody 5MIH9 in treating established infection, antibody was given at 24-hr intervals after intraperitoneal injection of bacteria. Delayed administration of antibody 5MIH9 was effective in reducing both the level and incidence of bacteremia.