A two-step sulfurization for efficient solution-phase synthesis of phosphorothioate oligonucleotides
A two-step sulfurization for efficient solution-phase synthesis of phosphorothioate oligonucleotides
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DOI：
10.1016/j.tetlet.2008.03.025
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发表时间：
2008-05-05
影响因子：
1.8
通讯作者：
van der Marel, Gijs A.
中科院分区：
文献类型：
--
作者：
Ghisaidoobe, Amar B. T.;de Koning, Martijn C.;van der Marel, Gijs A.
提出了一种固相法合成硫代磷酸酯寡核苷酸的方法，该方法避免了用色谱法纯化受保护的硫代磷酸酯中间体。在磷酰胺法中实施两步硫化协议，可以通过萃取有效地分离中间硫代磷酸酯。通过合成六聚硫代寡核苷酸片段证明了这种方法的可行性。(C) 2008 Elsevier Ltd版权所有。
A solution-phase approach for the synthesis of phosphorothioate oligonucleotides that circumvents the use of chromatographic purifications of protected phosphorothioate intermediates was developed. Implementation of a two-step sulfurization protocol in the phosphoramidite method allows efficient isolation of the intermediate phosphorothioates by extractions. The viability of this approach is demonstrated by the synthesis of a hexameric phosphorothioate oligonucleotide fragment. (C) 2008 Elsevier Ltd. All rights reserved.