Combining loose cell-attached stimulation and recording
Combining loose cell-attached stimulation and recording
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DOI：
10.1016/s0165-0270(00)00318-6
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发表时间：
2000-11-30
影响因子：
3
通讯作者：
Isope, P
中科院分区：
文献类型：
--
作者：
Barbour, B;Isope, P
许多突触生理学实验需要对突触前神经元进行选择性刺激。实现这一目标的单一方法没有一个是完全令人满意的。突触前全细胞或微电极记录提供了出色的控制，但建立突触连接神经元的这种记录可能非常耗时。最小的刺激提供了太少的信息关于突触前神经元的状态和兴奋。在松散的细胞连接结构中，刺激单个细胞并记录其动作电位是可能的，但大多数商用设备无法将刺激和记录结合起来。我们演示了同时使用一个特殊设计的膜片钳放大器的松散细胞连接刺激和动作电位记录。由于不需要严密的密封，该方法允许电极重复使用，从而能够快速筛选突触前细胞，同时选择性地刺激它们。(C) 2000 Elsevier Science B.V.版权所有
Many synaptic-physiology experiments require selective stimulation of presynaptic neurones. No single method for achieving this is entirely satisfactory. Presynaptic whole-cell or microelectrode recordings offer outstanding control, but establishing such recordings of synaptically-connected neurones can be very time consuming. Minimal stimulation provides too little information regarding the condition and excitation of the presynaptic neurone(s). In the loose cell-attached configuration, it is possible both to stimulate individual cells and to record their action potentials, but most commercially-available equipment will not enable Combining stimulation and recording. We demonstrate simultaneous loose cell-attached stimulation and recording of action potentials using a specially-designed patch-clamp amplifier. Since no tight seal is required, the method permits electrode re-use, thus enabling rapid screening of presynaptic cells while stimulating them selectively. (C) 2000 Elsevier Science B.V. All rights reserved.