TISSUE-CULTURE EVALUATION OF THE VIABILITY OF BLOOD VESSELS STORED BY REFRIGERATION
TISSUE-CULTURE EVALUATION OF THE VIABILITY OF BLOOD VESSELS STORED BY REFRIGERATION
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DOI：
10.1097/00000658-194903000-00006
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发表时间：
1949-01-01
影响因子：
9
通讯作者：
MERRILL, K
中科院分区：
文献类型：
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作者：
PEIRCE, EC;GROSS, RE;MERRILL, K
几十年来，实验外科医生一直在研究储存血管段的方法，并一直致力于在需要桥接动脉或静脉系统中的大间隙时使用这种储存的血管作为移植物的目标。将血管段储存在凡士林、潮湿空气、盐水、林格氏溶液和其他介质中，温度略高于冰点。6 7、8容器也被储存在冷冻状态下。2'3，16为了评估储存血管的存活能力，通常通过研究其组织学细节或通过将其一部分作为移植物植入并在活体动物的存活期内观察其来进行检查。用于移植的储存组织的活力仅在少数情况下通过组织培养方法进行了测试，8 '23但这种测试尚未应用于血管的研究。本文的目的是记录我们在组织培养研究中的观察结果，这些研究是在用各种方法储存的动脉段（动物和人）上进行的。
FOR SEVERAL DECADES experimental surgeons have been studying methods for the storage of vascular segments and have been working toward the goal of using such stored vessels as grafts whenever it is necessary to bridge a large gap in the arterial or venous system. Vascular segments have been stored in vaseline, moist air, saline, Ringer's solution, and other media, at temperatures slightly above freezing. 6 7, 8 Vessels have also been stored in a frozen state. 2'3, 16 To evaluate the viability of a stored vessel, examinations have generally been made by studying its histological detail or by implanting a portion of it as a graft and observing it during a survival period in a living animal. The viability of stored tissues destined for grafts has been tested by tissue-culture methods on only a few occasions, 8'23 but such tests have not been applied to the study of blood vessels. It is the purpose of the present communication to record our observations on tissue-culture studies which were made on arterial segments (animal and human) which were stored by various methods.**