Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis
Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis
复制标题
DOI：
10.1083/jcb.201910177
复制
发表时间：
2020-07-06
影响因子：
7.8
通讯作者：
Schneiter, Roger
中科院分区：
文献类型：
--
作者：
Choudhary, Vineet;El Atab, Ola;Schneiter, Roger
脂滴（LDs）是起源于内质网（ER）的脂肪储存细胞器。关于如何选择LD形成的位点以及该过程所需的蛋白质/脂质相对知之甚少。在这里，我们表明，由酵母三酰甘油（TAG）-脱氢酶Lro 1和Dga 1诱导的LD在由seipin（Fld 1）和二酰甘油（DAG）生产的调节剂Nem 1定义的离散ER亚结构域形成。Fld 1和Nem 1共定位于ER-LD接触位点。我们发现，Fld 1和Nem 1本地化ER亚域独立于彼此和LD，但都需要的亚域招募的TAG-酶和其他LD生物合成因子：Yft 2，Pex 30，Pet 10，和Erg 6。这些亚结构域在DAG中变得富集。我们的结论是，Fld 1和Nem 1都是必要的招募蛋白质ER亚域LD生物发生。
Lipid droplets (LDs) are fat storage organelles that originate from the endoplasmic reticulum (ER). Relatively little is known about how sites of LD formation are selected and which proteins/lipids are necessary for the process. Here, we show that LDs induced by the yeast triacylglycerol (TAG)-synthases Lro1 and Dga1 are formed at discrete ER subdomains defined by seipin (Fld1), and a regulator of diacylglycerol (DAG) production, Nem1. Fld1 and Nem1 colocalize to ER-LD contact sites. We find that Fld1 and Nem1 localize to ER subdomains independently of each other and of LDs, but both are required for the subdomains to recruit the TAG-synthases and additional LD biogenesis factors: Yft2, Pex30, Pet10, and Erg6. These subdomains become enriched in DAG. We conclude that Fld1 and Nem1 are both necessary to recruit proteins to ER subdomains where LD biogenesis occurs.