Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip
Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip
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DOI：
10.1016/j.aca.2008.12.025
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发表时间：
2009-02-23
影响因子：
6.2
通讯作者：
Gu, Man Bock
中科院分区：
文献类型：
--
作者：
Kim, Yeon Seok;Niazi, Javed H.;Gu, Man Bock
使用固定在金叉指阵列（IDA）电极芯片上的单链DNA适体开发了用于土霉素（OTC）检测的电化学传感系统。采用以高亲和力与 OTC 结合的高度特异性 ssDNA 适体来区分其他四环素 (TC)，例如强力霉素 (DOX) 和四环素 JET)。将硫醇修饰的适配体固定在金电极芯片上作为目标分子的生物识别元件，通过循环伏安法（CV）和方波伏安法（SWV）评估适配体与目标分子相互作用产生的电化学信号。通过亚铁氰化物和铁氰化物之间的氧化还原反应产生的电子流来分析由于结合的 OTC、DOX 或 TET 的干扰而导致的电流减小。开发的 OTC EC 生物传感器的特异性与结构相似的抗生素（DOX 和 TET）有很大区别。动态范围确定为半对数坐标中 OTC 浓度的 1-100 nM。 (C) 2008 Elsevier B.V. 保留所有权利。
An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline JET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates. (C) 2008 Elsevier B.V. All rights reserved.