Cytochrome P4501A (CYP1A) in killifish (Fundulus heteroclitus):: Heritability of altered expression and relationship to survival in contaminated sediments
Cytochrome P4501A (CYP1A) in killifish (Fundulus heteroclitus):: Heritability of altered expression and relationship to survival in contaminated sediments
复制标题
DOI：
10.1093/toxsci/68.1.69
复制
发表时间：
2002-07-01
影响因子：
3.8
通讯作者：
Di Giulio, RT
中科院分区：
文献类型：
--
作者：
Meyer, JN;Nacci, DE;Di Giulio, RT
先前的研究表明，在弗吉尼亚州伊丽莎白河杂酚油污染的场地栖息的鱼（底栖）表现出细胞色素P4501 A（CYP 1A）蛋白的表达和活性的诱导后，暴露于典型的CYP 1A诱导化学品。我们的特点是CYP 1A的第一，第二，第三代实验室提出的野生伊丽莎白河鳉鱼的后代暴露于沉积物污染的网站，以及典型的多环芳烃（PAH）型CYP 1A诱导剂β-萘甲酮（BNF）和3-甲基胆蒽（3-MC）的响应。伊丽莎白河后代的反应进行了比较，从两个参考网站（国王溪，弗吉尼亚州和罗素溪，北卡罗来纳州）的？与野生伊丽莎白河鳉鱼，第一代胚胎和幼虫是难治的CYP 1A诱导。然而，在3岁的第一代成年人，以及第二代和第三代鱼，观察到的反应是更接近于在参考网站的鱼。我们认为，改变CYP 1A反应的模式，伊丽莎白河？此外，我们研究了低CYP 1A活性（以卵EROD活性测量）与伊丽莎白河沉积物孔隙水中存活率增加相关的假设;我们的结果不支持这一假设。
Previous research has shown that killifish (Fundulus heteroclitus) inhabiting a creosote-contaminated site on the Elizabeth River in Virginia exhibit little induction of cytochrome P4501A (CYP1A) protein expression and activity upon exposure to typical CYP1A-inducing chemicals. We characterized the CYP1A response of first, second, and third generation laboratory-raised offspring of feral Elizabeth River killifish to exposure to sediments from the contaminated site as well as the prototypical polycyclic aromatic hydrocarbon (PAH)-type CYP1A inducers beta-naphthoflavone (BNF) and 3-methylcholanthrene (3-MC). The Elizabeth River offspring's responses were compared to those of offspring of killifish from two reference sites (King's Creek, Virginia, and Russell Creek, North Carolina). As with feral Elizabeth River killifish, the first generation embryos and larvae were refractory to CYP1A induction. However, the response observed in 3-year-old first generation adults, as well as with second and third generation fish, was much closer to that observed in reference-site fish. We suggest that the pattern of altered CYP1A response in Elizabeth River killifish, while persistent and heritable for one generation, is mostly nongenetically based. Additionally, we investigated the hypothesis that low CYP1A activity (measured as in ovo EROD activity) would correlate to increased survival in Elizabeth River sediment pore water; this hypothesis was not supported by our results.