Neurites of period-expressing PDH cells in the fly's optic lobe exhibit circadian oscillations in morphology.
Neurites of period-expressing PDH cells in the fly's optic lobe exhibit circadian oscillations in morphology.
复制标题
果蝇视叶中表达周期的 PDH 细胞的神经突在形态上表现出昼夜节律振荡。
DOI：
10.1111/j.1460-9568.1997.tb01537.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Meinertzhagen,IA
中科院分区：
文献类型：
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作者：
Pyza,E;Meinertzhagen,IA
昼夜节律已在果蝇视叶的“外侧神经元”和最外层神经纤维或层的细胞中的 period (per) 基因的表达中得到体现。一些外侧神经元还表现出 PDH 肽样免疫反应性，在整个视叶中广泛分布。使用家蝇共聚焦显微镜，我们分析了 PDH 神经突静脉曲张的大小和间距，以及可能表现出昼夜节律的肽释放位点。在持续黑暗的主观白天，与主观夜间相比，存在更少、更大的静脉曲张。内源性节律被昼夜循环和连续光照条件下发生的光照掩盖。我们的研究结果表明，PDH 神经突从起搏器传递昼夜节律信息，它们可以通过周期性释放其肽来调节椎板中描述的昼夜节律。
Circadian rhythms have been shown both in the expression of theperiod (per) gene in ‘lateral neurons’ and in cells of the outermost neuropil, or lamina, of the fly's optic lobe. Some lateral neurons also exhibit PDH peptide‐like immunoreactivity, arborizing widely throughout the optic lobe. Using confocal microscopy in the housefly, we analysed the size and spacing of PDH neurite varicosities, sites of possible peptide release exhibiting circadian rhythmicity. During the subjective day in constant darkness, there were fewer, larger varicosities than during subjective night. The endogenous rhythm was masked by the light exposure that occurred under a day‐night cycle and continuous light conditions. Our findings indicate that PDH neurites convey circadian information out from the pacemaker, where they could regulate the circadian rhythms that have been described in the lamina, possibly via cyclical release of their peptide.