Fe3O4@Au SERS tags-based lateral flow assay for simultaneous detection of serum amyloid A and C-reactive protein in unprocessed blood sample
Fe3O4@Au SERS tags-based lateral flow assay for simultaneous detection of serum amyloid A and C-reactive protein in unprocessed blood sample
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DOI：
10.1016/j.snb.2020.128350
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发表时间：
2020-10-01
影响因子：
8.4
通讯作者：
Wang, Shengqi
中科院分区：
文献类型：
--
作者：
Liu, Xiaoxian;Yang, Xingsheng;Wang, Shengqi
通过侧流免疫测定（LFA）条准确检测未处理血液中的多种生物标志物仍然具有挑战性。在此，我们报道了一种新型的基于表面增强拉曼散射（Sers）的LFA条带，通过使用功能化的Fe3O4@Au磁性纳米颗粒（MNP），用于超灵敏和同时分析双重感染生物标志物。抗体修饰的Fe3O4@Au纳米粒子被设计为Sers纳米标签和有效的富集工具。通过Fe3O4@Au纳米标签从血液样品中快速捕获和富集目标感染生物标志物。在洗涤以去除杂质后，Fe3O4@Au纳米标签充当高性能Sers报告物，用于定量检测LFA条带中的目标。通过这种策略，提出的基于Fe3O4@Au的LFA允许对未处理血液样品中的目标感染生物标志物进行定量分析，血清淀粉样蛋白A（SAA）和C反应蛋白（CRP）的检测限分别为0.1 ng/mL和0.01 ng/mL。通过比较，SAA和CRP的拟议测定是100倍和1000倍以上的敏感性比标准胶体金条，通过使用相同的抗体，分别。这种磁性Sers条带还表现出优异的稳定性、特异性和对复杂样品的选择性，可以用作传染病诊断的有效工具。
Accurate detection of mutiple biomarkers in unprocessed blood via lateral flow immunoassay (LFA) strips remains challenging. Herein, we reported a novel surface-enhanced Raman scattering (SERS)-based LFA strip for ultrasensitive and simultaneous analysis of dual infection biomarkers by using functionalized Fe3O4@Au magnetic nanoparticles (MNPs). Antibody-modified Fe3O4@Au MNPs were designed as SERS nanotags and efficient enrichment tool. The target infection biomarkers were quickly captured and enriched from the blood sample by Fe3O4@Au nanotags. After washing to remove impurities, Fe3O4@Au nanotags acted as high-performance SERS reporters for quantitative detection of the target in the LFA strip. Through this strategy, the proposed Fe3O4@Au based LFA allowed quantified analysis of target infection biomarkers in unprocessed blood samples with the limits of detection of 0.1 ng/mL and 0.01 ng/mL for serum amyloid A (SAA) and C-reactive protein (CRP), respectively. By comparison, the proposed assay for SAA and CRP was 100-fold and 1000-fold more sensitive than standard colloidal gold strip by using the same antibodies, respectively. This magnetic SERS strip also exhibited excellent stability, specificity, and selectivity for complex samples and could be used as an effective tool for infectious disease diagnostics.