Expression of hypoxia-inducible factor (HIF)-1α is associated with vascular endothelial growth factor expression and tumour anglogenesis in human oesophageal squamous cell carcinoma
Expression of hypoxia-inducible factor (HIF)-1α is associated with vascular endothelial growth factor expression and tumour anglogenesis in human oesophageal squamous cell carcinoma
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DOI：
10.1016/j.ejca.2004.04.035
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发表时间：
2004-08-01
影响因子：
8.4
通讯作者：
Chayama, K
中科院分区：
文献类型：
--
作者：
Kimura, S;Kitadai, Y;Chayama, K
本研究的目的是探讨食管鳞状细胞癌中缺氧诱导因子（HIF）-1 α表达、血管内皮生长因子（VEGF）表达和肿瘤血管分布之间的关系。检测了两种食管鳞癌细胞系（TE 2、TE 3）和82例食管鳞癌手术标本中HIF-1 α和VEGF的表达。在两种细胞系中，HIF-1 α蛋白和VEGF mRNA的水平在缺氧条件下增加。82例肿瘤标本中的32例（39%）在癌细胞的细胞核和/或细胞质中显示高水平的HIF-1 α免疫反应性。HIF-1a表达与静脉浸润、VEGF表达及微血管密度无明显相关性。在47例术前未接受化疗的患者中，HIF-1 α高表达肿瘤的预后明显差于F[IF-1 α]低表达肿瘤的预后。这些结果表明，HIF-1 α和VEGF的表达是食管鳞状细胞癌生存的重要决定因素。(C)2004爱思唯尔有限公司保留所有权利。
The purpose of this study was to examine the relationship between hypoxia inducible factor (HIF)-1alpha expression, vascular endothelial growth factor (VEGF) expression, and tumour vascularity in squamous cell carcinoma of the oesophagus. Expression of HIF-1alpha and VEGF was examined in two oesophageal squamous cell carcinoma cell lines (TE2, TE3) and 82 archival surgical specimens of human oesophageal squamous cell carcinoma tissue. In both cell lines, the levels of HIF-1alpha protein and VEGF mRNA were increased under hypoxic conditions. Thirty-two of the 82 (39%) tumour specimens showed high levels of HIF-1alpha immunoreactivity in the nuclei and/or cytoplasm of cancer cells. HIF-1a expression cot-related significantly with venous invasion, VEGF expression, and microvessel density. Among the 47 patients who did not receive pre-operative chemotherapy, the outcome of those with high HIF-1alpha-expressing tumours was significantly poorer than that of those with low F[IF-1alpha-expressing turnours. These results suggest that HIF-1alpha and VEGF expression are important determinants of survival in squamous cell carcinoma of the oesophagus. (C) 2004 Elsevier Ltd. All rights reserved.