Differential expression of Prominin-1 (CD133) and Prominin-2 in major cephalic exocrine glands of adult mice
Differential expression of Prominin-1 (CD133) and Prominin-2 in major cephalic exocrine glands of adult mice
复制标题
DOI：
10.1007/s00418-007-0334-2
复制
发表时间：
2007-11-01
影响因子：
2.3
通讯作者：
Corbeil, Denis
中科院分区：
文献类型：
--
作者：
Jaszai, Jozsef;Janich, Peggy;Corbeil, Denis
主要的头部外分泌腺具有许多形态和功能特征，因此在某些自身免疫性和遗传性疾病中可同时受到影响，从而导致腺体功能低下。这些外分泌腺的表型特征不仅是一个有趣的生物学问题，而且可能具有相当大的临床相关性。因此，主要的唾液腺和泪腺可能成为未来基于细胞的再生/组织工程治疗方法的潜在对象。在本研究中，我们描述了干细胞和祖细胞标记物Prominin-1及其类似物Prominin-2在成年小鼠颌下腺、主要舌下腺和腮腺三对主要唾液腺中的表达。我们还记录了它们在眼睑(眼睑)的眶外泪腺和眉板腺中的表达。我们的分析表明，小鼠Prominin-1和Prominin-2在这些主要的头外分泌器官中存在差异表达。Prominin-1的表达与导管系统有关，而Prominin-2的表达主要(但不限于)在这些器官的腺泡间，不同腺体之间存在显著差异。最后，我们报告了Prominin-2，像Prominin-1一样，被释放到人类唾液中，与微小的膜颗粒相关，具有未来诊断应用的潜力。
The major cephalic exocrine glands share many morphological and functional features and so can be simultaneously affected in certain autoimmune- and inherited disorders leading to glandular hypofunction. Phenotypic characterization of these exocrine glands is not only an interesting biological issue, but might also be of considerable clinical relevance. The major salivary and lacrimal glands might therefore be potential subjects of future cell-based regenerative/tissue engineering therapeutic approaches. In the present study, we described the expression of the stem and progenitor cell marker Prominin-1 and those of its paralogue, Prominin-2, in the three pairs of major salivary glands, i.e., submandibular-, major sublingual-, and parotid glands in adult mice. We have also documented their expression in the extraorbital lacrimal and meibomian glands (Glandulae tarsales) of the eyelid (Palpebra). Our analysis revealed that murine Prominin-1 and Prominin-2 were differentially expressed in these major cephalic exocrine organs. Expression of Prominin-1 was found to be associated with the duct system, while Prominin-2 expression was mostly, but not exclusively, found in the acinar compartment of these organs with marked differences among the various glands. Finally, we report that Prominin-2, like Prominin-1, is released into the human saliva associated with small membrane particles holding the potential for future diagnostic applications.