Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015).
Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015).
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DOI：
10.1186/s13071-016-1607-1
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发表时间：
2016-06-01
影响因子：
3.2
通讯作者：
Gasser RB
中科院分区：
文献类型：
--
作者：
Koehler AV;Haydon SR;Jex AR;Gasser RB
在一个监测澳大利亚维多利亚州墨尔本集水区的病原体的长期计划中，我们在四年(2011年7月至2015年11月)期间从九个蓄水区的各种动物的粪便样本中检测到隐孢子虫和贾第虫，并对其进行了基因特征分析。对隐孢子虫核糖体RNA小亚基(SSU)和60 kDa糖蛋白(Gp60)基因以及贾第虫磷酸丙糖异构酶(TPI)基因的部分片段进行了基于聚合酶链式反应的单链构象多态性(SSCP)和系统发育分析。隐孢子虫的流行率为1.62%(69/4256)；pSSU定义了25种不同的序列类型，gp60定义了6种不同的序列类型，分别代表人隐孢子虫(Ib亚型IbA10G2)、楔形隐孢子虫(Vb亚型VbA26和VbA25)、犬隐孢子虫、费耶里隐孢子虫、大孔隐孢子虫、微小隐孢子虫、兰氏隐孢子虫和隐孢子虫。“鸭”型、猪链霉菌和泛源链霉菌以及12种新的SSU序列类型。贾第虫的患病率为0.31%(4,256个样本中有13个)；定义的所有三种不同的TPI序列类型都代表十二指肠贾第虫的A组合。在这里描述的34种序列类型(基因类型)中，以前从人类中分别记录到了5种隐孢子虫和1种贾第虫。隐孢子虫和贾第鞭毛虫的SSu(n = 12)、gp60(n = 4)和Tpi(n = 1)的新基因型别目前尚不清楚。未来的工作将继续监测隐孢子虫和贾第鞭毛虫在这些集水区动物中的流行情况，并将调查扩大到人类。本文报道的核苷酸序列可在GenBank数据库中获得，登录号为：KU531647-KU531718。本文的在线版本(doi：10.1186/s13071-016-1607-1)包含补充材料，授权用户可以使用。
In a long-term program to monitor pathogens in water catchments serving the City of Melbourne in the State of Victoria in Australia, we detected and genetically characterised Cryptosporidium and Giardia in faecal samples from various animals in nine water reservoir areas over a period of 4 years (July 2011 to November 2015). This work was conducted using PCR-based single-strand conformation polymorphism (SSCP) and phylogenetic analyses of portions of the small subunit of ribosomal RNA (SSU) and 60 kDa glycoprotein (gp60) genes for Cryptosporidium, and triose-phosphate isomerase (tpi) gene for Giardia. The prevalence of Cryptosporidium was 1.62 % (69 of 4,256 samples); 25 distinct sequence types were defined for pSSU, and six for gp60 which represented C. hominis (genotype Ib - subgenotype IbA10G2), C. cuniculus (genotype Vb - subgenotypes VbA26, and VbA25), and C. canis, C. fayeri, C. macropodum, C. parvum, C. ryanae, Cryptosporidium sp. “duck” genotype, C. suis and C. ubiquitum as well as 12 novel SSU sequence types. The prevalence of Giardia was 0.31 % (13 of 4,256 samples); all three distinct tpi sequence types defined represented assemblage A of G. duodenalis. Of the 34 sequence types (genotypes) characterized here, five and one have been recorded previously for Cryptosporidium and Giardia, respectively, from humans. Novel genotypes of Cryptosporidium and Giardia were recorded for SSU (n = 12), gp60 (n = 4) and tpi (n = 1); the zoonotic potential of these novel genotypes is presently unknown. Future work will continue to monitor the prevalence of Cryptosporidium and Giardia genotypes in animals in these catchments, and expand investigations to humans. Nucleotide sequences reported in this paper are available in the GenBank database under accession nos. KU531647–KU531718. The online version of this article (doi:10.1186/s13071-016-1607-1) contains supplementary material, which is available to authorized users.