Evidence that a single peptide-MHC complex on a target cell can elicit a cytolytic T cell response
Evidence that a single peptide-MHC complex on a target cell can elicit a cytolytic T cell response
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DOI：
10.1016/s1074-7613(00)80483-5
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发表时间：
1996-06-01
期刊：
影响因子：
32.4
通讯作者：
Eisen, HN
中科院分区：
文献类型：
--
作者：
Sykulev, Y;Joo, M;Eisen, HN
使用化学均质的放射性标记的高比活度肽（I-125-QLSPYPFDL，3.5 × 10（18）cpm/摩尔），我们表明，在肽浓度（5 pM）下，通过细胞溶解性T淋巴细胞（CTL）克隆引起靶细胞的半数最大溶解，所述细胞溶解性T淋巴细胞（CTL）克隆识别与L（d）（一种I类MHC蛋白）结合的肽，每个靶细胞平均只有3个肽分子被L（d）结合。从L（d）在靶细胞上的分布，我们认为每个靶细胞的单个肽-MHC复合物可以触发T细胞溶细胞反应的激活。
Using a chemically homogeneous radiolabeled peptide of high specific activity (I-125-QLSPYPFDL, 3.5 x 10(18) cpm per mole) we show that at a peptide concentration (5 pM) causing half-maximal lysis of target cells by a cytolytic T lymphocyte (CTL) clone that recognizes the peptide in association with L(d), a class I MHC protein, only 3 peptide molecules on average are bound by L(d) per target cell. From the distribution of L(d) on the target cells, we suggest that a single peptide-MHC complex per target cell can trigger activation of the T cell cytolytic response.