Axonal transport proteins and depressive like behavior, following Chronic Unpredictable Mild Stress in male rat
Axonal transport proteins and depressive like behavior, following Chronic Unpredictable Mild Stress in male rat
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DOI：
10.1016/j.physbeh.2018.04.029
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发表时间：
2018-10-01
影响因子：
2.9
通讯作者：
Niknazar, Somayeh
中科院分区：
文献类型：
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作者：
Bakhtiarzadeh, Fatemeh;Nahavandi, Arezo;Niknazar, Somayeh
背景：抑郁症是一种常见的心境障碍，长期以来一直被认为是全球残疾的主要原因。慢性压力与包括重度抑郁症在内的各种精神疾病的发展有关。压力可以诱发抑郁样症状，并启动大脑中的神经退行性过程。抑郁症的神经退行性理论认为受损的轴突运输是神经存活的负面因素。轴突运输是正常神经元功能的关键机制，在轴突生长、神经递质分泌、正常线粒体功能和神经存活中发挥关键作用。方法和材料：为探讨应激对抑郁的影响，本研究采用强迫游泳实验（FST）、酶联免疫吸附试验（ELISA）测定血浆皮质酮水平、海马三个基因mRNA表达结果：与对照组（非应激组）相比，应激组大鼠海马神经生长因子（NGF）、驱动蛋白（kinesin）和动力蛋白（dynein）基因的表达显著降低（p < 0.05）。结论：海马神经元退行性变与应激性抑郁行为的发生密切相关，其机制可能与两种最重要的轴突运输蛋白kinesin和dynein表达降低有关。
Background: A common mood disorder, depression has long been considered a leading cause of disability worldwide. Chronic stress is involved in the development of various psychiatric diseases including major depressive disorder. Stress can induce depressive-like symptoms and initiate neurodegenerative processes in the brain. The neurodegenerative theory of depression holds impaired axonal transport as a negative factor in neural survival. Axonal transport is a critical mechanism for normal neuronal function, playing crucial roles in axon growth, neurotransmitter secretion, normal mitochondrial function and neural survival.Methods and materials: To investigate the effects of stress-induced depression, in the present study, we evaluated behavior by forced swimming test (FST), corticosterone plasma level by ELISA assay, hippocampal mRNA expression of three genes (NGF, kinesin and dynein) via real-time PCR and hippocamp count by Nissl staining in male Wistar rats.Results: Our data demonstrated a significant decrease in the expression of NGF, kinesin and dynein genes in CUMS groups compared to the control group (non-stressed) (p < 0.05). CUMS also caused an elevation in immobility time and corticosterone plasma level in the stressed group compared to the controls (p < 0.01 and p < 0.05, respectively).Conclusion: The results suggested that the possibility of stress-induced depressive behavior associated with hippocampal neurodegeneration process is correlated with a low expression of kinesin and dynein, the two most important proteins in axonal transport.