Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation.
Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation.
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DOI：
10.7554/elife.58609
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发表时间：
2021-06-04
期刊：
影响因子：
7.7
通讯作者：
Winter SE
中科院分区：
文献类型：
--
作者：
Hughes ER;Winter MG;Alves da Silva L;Muramatsu MK;Jimenez AG;Gillis CC;Spiga L;Chanin RB;Santos RL;Zhu W;Winter SE
肠道相关微生物群落的组成在肠道炎症期间发生变化，包括肠杆菌科种群的扩张。炎症期间微生物群变化的机制尚未完全了解。在这里，我们分析了以前发表的宏基因组数据集，重点是微生物的氢代谢。与非炎症条件下发现的群落相比，炎症小鼠肠道和炎症性肠病患者中的细菌基因组包含更多编码预测的氢利用氢化酶的基因。为了验证这些发现，我们研究了大肠杆菌，一个代表性的肠杆菌科，在小鼠结肠炎模型的氢代谢。E.缺乏氢化酶-1和氢化酶-2的大肠杆菌突变体在发炎的盲肠和结肠的定殖期间显示出降低的适应性。分子氢的利用部分依赖于炎症衍生的电子受体的呼吸。这项工作突出了氢化酶在非感染性结肠炎背景下对肠道微生物群改变的贡献。
The composition of gut-associated microbial communities changes during intestinal inflammation, including an expansion of Enterobacteriaceae populations. The mechanisms underlying microbiota changes during inflammation are incompletely understood. Here, we analyzed previously published metagenomic datasets with a focus on microbial hydrogen metabolism. The bacterial genomes in the inflamed murine gut and in patients with inflammatory bowel disease contained more genes encoding predicted hydrogen-utilizing hydrogenases compared to communities found under non-inflamed conditions. To validate these findings, we investigated hydrogen metabolism of Escherichia coli, a representative Enterobacteriaceae, in mouse models of colitis. E. coli mutants lacking hydrogenase-1 and hydrogenase-2 displayed decreased fitness during colonization of the inflamed cecum and colon. Utilization of molecular hydrogen was in part dependent on respiration of inflammation-derived electron acceptors. This work highlights the contribution of hydrogenases to alterations of the gut microbiota in the context of non-infectious colitis.