Detection of Biospecific Interaction during the First Dimension Electrophoresis in Crossed Immunoelectrophoresis *
Detection of Biospecific Interaction during the First Dimension Electrophoresis in Crossed Immunoelectrophoresis *
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交叉免疫电泳第一维电泳过程中生物特异性相互作用的检测*
DOI：
10.1111/j.1365-3083.1975.tb03820.x
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发表时间：
1975
影响因子：
3.7
通讯作者：
J. Ramlau
中科院分区：
文献类型：
--
作者：
T. Bøg‐Hansen;O. Bjerrum;J. Ramlau
人血清蛋白交叉免疫电泳的第一维凝胶中含有凝集素。Con A在第一次电泳中导致大多数糖蛋白的阻滞，并且可以检测到不同形式的单个糖蛋白。欧洲镰刀凝集素使大多数蛋白质具有较高的迁移速度。美洲商陆丝裂原与血清糖蛋白无相互作用，但与血清脂蛋白有相互作用。该方法允许密切研究相互作用组分，确定结合特异性，并检测次要相互作用组分，包括异质分子形式。
Lectins were included in the first dimension gel in crossed immunoelectrophoresis of human serum proteins. Con A resulted in a retardation of most glycoproteins during the first electrophoresis and heterogeneous forms of individual glycoproteins could be detected. Ulex europeus lectin gave most of the proteins a higher migration velocity. Pokeweed mitogen did not interact with serum glycoproteins, but it did with serum lipoproteins. The method allows a close study of interacting components, determination of binding specificities, and detection of minor interacting components, including heterogeneous molecular forms.