Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes
Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes
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DOI：
10.1016/j.elecom.2006.03.008
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发表时间：
2006-05-01
影响因子：
5.4
通讯作者：
Gorton, Lo
中科院分区：
文献类型：
--
作者：
Pita, Marcos;Shleev, Sergey;Gorton, Lo
裸金电极和巯基修饰的金电极与不同来源的真菌漆酶之间的无介体（直接）电子转移已被证明。担子菌毛栓菌酶的电化学活性。利用循环伏安法和光谱电化学法可以清楚地观察到好氧和厌氧条件下的赭色栓菌（Trametes ochracea）和Cerrena maxima。T.氨基苯硫酚修饰金电极固定化多毛菌漆酶，从+625 mV（相对于NHE）开始，并显示了固定在裸电极和硫醇修饰电极上的酶的生物电催化的差异。结果表明，过氧化氢是真菌漆酶修饰金电极上氧电还原的产物之一，而T.固定在巯基修饰的金电极上的多毛类漆酶。因此，两种不同的机制吸附在裸电极和巯基修饰电极上的真菌漆酶的氧电还原的假说提出。(c)2006 Elsevier B.V.保留所有权利。
Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirstita. Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified gold electrodes., starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygen electroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed. (c) 2006 Elsevier B.V. All rights reserved.