Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge
Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge
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日粮补充 L-精氨酸可改善大肠杆菌脂多糖攻击后断奶猪的肠道功能
DOI：
10.5713/ajas.2009.90100
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发表时间：
2009-12-01
期刊：
影响因子：
--
通讯作者：
Wang, Junjun
中科院分区：
文献类型：
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作者：
Liu, Yulan;Han, Jie;Wang, Junjun
本研究旨在探讨L-精氨酸（Arg）能否改善大肠杆菌脂多糖（LPS）攻击后断奶仔猪的肠道功能。治疗包括：i）未攻击的对照（对照，饲喂对照饮食并用无菌盐水注射的猪）; ii）LPS攻击的对照（LPS，饲喂相同对照饮食并用大肠杆菌LPS注射攻击的猪）; iii）LPS+0.5%Arg（饲喂0.5%Arg饮食并用LPS攻击的猪）;和iv）LPS+1.0%Arg（饲喂1.0%Arg饮食并用LPS攻击的猪）。第16天，分别给予LPS和生理盐水。在LPS攻击后2 h口服D-木糖，并在LPS攻击后3 h收集血液样品。攻毒后6 h，处死猪，收集肠粘膜样品。补充Arg可减轻LPS引起的肠道消化和屏障功能的损害，如回肠乳糖酶活性、十二指肠和回肠二胺氧化酶活性的增加所示（p<0.05）。精氨酸还能防止LPS刺激引起的空肠丙二醛含量的升高和回肠超氧化物歧化酶活性的降低（p<0.05）。补充精氨酸后，空肠一氧化氮水平和诱导型一氧化氮合酶活性也显著提高（p<0.05）。这些结果表明，补充精氨酸对减轻LPS攻击引起的肠道功能损害具有有益作用。
This study was conducted to determine whether L-arginine (Arg) supplementation could improve intestinal function in weaned pigs after an Escherichia coli lipopolysaccharide (LPS) challenge. Treatments included: i) non-challenged control (CONTR, pigs fed a control diet and injected with sterile saline); ii) LPS-challenged control (LPS, pigs fed the same control diet and challenged by injection with Escherichia coli LPS); iii) LPS+0.5% Arg (pigs fed a 0.5% Arg diet and challenged with LPS); and iv) LPS+1.0% Arg (pigs fed a 1.0% Arg diet and challenged with LPS). On d 16, pigs were administrated with LPS or sterile saline. D-xylose was orally administrated at 2 h following LPS challenge, and blood samples were collected at 3 h following LPS challenge. At 6 h post-challenge, pigs were sacrificed and intestinal mucosa samples were collected. Supplementation of Arg attenuated LPS-induced damage in gut digestive and barrier functions, as indicated by an increase in ileal lactase activity, and duodenal and ileal diamine oxidase activities (p<0.05). Arg administration also prevented the increase of jejunal malondialdehyde content and the decrease of ileal superoxide dismutase activity by LPS challenge (p<0.05). Furthermore, the jejunal nitric oxide level and inducible nitric oxide synthase activity were also improved after Arg supplementation (p<0.05). These results indicate that Arg supplementation has beneficial effects in alleviating the impairment of gut function induced by LPS challenge.