THE PCL2 (ORFD)-PHO85 CYCLIN-DEPENDENT KINASE COMPLEX - A CELL-CYCLE REGULATOR IN YEAST
THE PCL2 (ORFD)-PHO85 CYCLIN-DEPENDENT KINASE COMPLEX - A CELL-CYCLE REGULATOR IN YEAST
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DOI：
10.1126/science.7973731
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发表时间：
1994-11-25
期刊：
影响因子：
56.9
通讯作者：
ANDREWS, B
中科院分区：
文献类型：
--
作者：
MEASDAY, V;MOORE, L;ANDREWS, B
细胞周期蛋白依赖性激酶（cdk）复合物是所有真核生物细胞周期进程的必要激活剂。哺乳动物细胞中有多个cdk参与细胞周期调节，而酵母细胞周期主要由单个cdk CDC28的活性控制。对推测的G(1)周期蛋白PCL2 （ORFD）的分析发现了第二个周期蛋白-cdk复合物，该复合物有助于酵母的细胞周期进程。PCL2在体内和体外与cdk PHO85相互作用，形成具有G(1)周期活性的激酶复合物。在Start过渡受损的遗传条件下，PHO85及其相关的细胞周期蛋白亚基对细胞周期承诺至关重要。由于PHO85和另一种细胞周期蛋白样分子PHO80也参与无机磷酸盐代谢，这种cdk酶可能将对营养条件的反应与细胞周期结合起来。
Cyclin-dependent kinase (cdk) complexes are essential activators of cell cycle progression in all eukaryotes. In contrast to mammalian cells, in which multiple cdk's contribute to cell cycle regulation, the yeast cell cycle is largely controlled by the activity of a single cdk, CDC28. Analysis of the putative G(1) cyclin PCL2 (ORFD) identified a second cyclin-cdk complex that contributes to cell cycle progression in yeast. PCL2 interacted with the cdk PHO85 in vivo and in vitro and formed a kinase complex that had G(1)-periodic activity. Under genetic conditions in which the Start transition was compromised, PHO85 and its associated cyclin subunits were essential for cell cycle commitment. Because PHO85 and another cyclin-like molecule, PHO80, also take part in inorganic phosphate metabolism, this cdk enzyme may integrate responses to nutritional conditions with the cell cycle.