Digestive enzyme activities during pejerrey (Odontesthes bonariensis) ontogeny
Digestive enzyme activities during pejerrey (Odontesthes bonariensis) ontogeny
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DOI：
10.1016/j.aquaculture.2020.735151
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发表时间：
2020-07
期刊：
影响因子：
4.5
通讯作者：
D. I. Pérez-Sirkin;M. Solovyev;T. Delgadin;Javier E. Herdman;L. Miranda;G. Somoza;P. Vissio;E. Gisbert
中科院分区：
文献类型：
--
作者：
D. I. Pérez-Sirkin;M. Solovyev;T. Delgadin;Javier E. Herdman;L. Miranda;G. Somoza;P. Vissio;E. Gisbert
评估和完善鱼的幼体和早期幼鱼阶段的营养需求的第一步是研究消化系统功能的个体发育。这些研究与感兴趣物种的生态和解剖学知识相结合，为应对水产养殖的主要挑战之一奠定了基础：促进幼虫生长和存活。考虑到这一点，主要消化（胰腺和肠道）酶活性的变化，在幼虫发育过程中的agastric南美pejerrey（Odontesthes bonariensis）在本工作中进行了描述。蛋白质、脂肪和碳水化合物水解的消化酶从孵化后第一周开始存在（全长6.85 ± 0.07 mm，TL）。胰蛋白酶、胰凝乳蛋白酶和碱性蛋白酶活性的变化表明，白颈沙蚕外分泌胰腺在出膜后2周（9.22 ± 0.17 mm TL）达到功能性发育。有趣的是，α-淀粉酶和麦芽糖酶的总活性随着发育而逐渐增加，这表明在饲养方案中逐渐加入膳食碳水化合物可能具有节省蛋白质的效果，以及为pejerrey的发育和生长获得能量的廉价而快速的方法。肠酶的分析表明，典型的细胞内和管腔蛋白质消化之间的转变，发生在幼虫发育过程中胃的物种不发生在pejerrey，表明在agastric物种细胞内蛋白质消化起着重要的作用，在幼虫发育过程中的管腔消化相比。相反，胃的物种，我们的研究结果表明，碱性磷酸酶的亮氨酸氨肽酶的比例，用于评估肠道成熟的agastric物种是不推荐的，和其他参数时，应测量评估从这组物种的鱼苗的成熟过程。
The first step for assessing and refining the nutritional requirements during the larval and early juvenile stages of a fish is the study of the ontogeny of digestive system functionality. The combination of these studies with ecological and anatomical knowledge of the species of interest establishes the base for facing one of the major aquaculture challenges: promoting larvae growth and survival. Considering this, changes in the activity of the main digestive (pancreatic and intestinal) enzymes during larval development of the agastric South America pejerrey (Odontesthes bonariensis) were described in the present work. Digestive enzymes for protein, lipid, and carbohydrate hydrolysis were present from the first-week post-hatching (6.85 ± 0.07 mm total length, TL). Changes in the activity of trypsin, chymotrypsin, and alkaline protease indicated that the exocrine pancreas in pejerrey achieves its functional development at the 2nd week post-hatching (9.22 ± 0.17 mm TL). Interestingly, α-amylase and maltase total activity progressively increased over development, suggesting that gradual incorporation of dietary carbohydrates in a feeding protocol may have a protein-sparing effect, as well as a cheap and fast way to obtain energy for the development and growth of pejerrey. The analysis of intestinal enzymes revealed that the typical shift between intracellular and luminal protein digestion that occurs during larval development in gastric species does not take place in pejerrey, indicating that in agastric species intracellular protein digestion plays a major role in comparison to luminal digestion during larval development. Contrary to gastric species, our results suggest that the ratio of alkaline phosphatase to leucine-aminopeptidase for evaluating gut maturation in agastric species is not recommended, and other parameters should be measured when evaluating the maturation process in fish larvae from this group of species.