Identification and characterization of globin genes from two lepidopteran insects, Bombyx mori and Samia cynthia ricini.
Identification and characterization of globin genes from two lepidopteran insects, Bombyx mori and Samia cynthia ricini.
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DOI：
10.1016/j.gene.2008.11.004
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发表时间：
2009-02
期刊：
影响因子：
3.5
通讯作者：
S. Kawaoka;S. Katsuma;Y. Meng;Nobumitsu Hayashi;K. Mita;T. Shimada
中科院分区：
文献类型：
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作者：
S. Kawaoka;S. Katsuma;Y. Meng;Nobumitsu Hayashi;K. Mita;T. Shimada
我们描述了两个鳞翅目昆虫，家蚕（Bmglobin）和蓖麻蚕（Scglobin）的血红蛋白样基因的特性。Bmglobin和Scglobin被预测为细胞内蛋白质并且含有血红素和氧结合所需的氨基酸。两种鳞翅目昆虫球蛋白，特别是Bmglobin的表达谱与其他昆虫球蛋白不同。尽管其他昆虫球蛋白主要与气管系统相关，但Bmglobin几乎仅在马氏管中表达，并且在脂肪体中检测到Scglobin的最强信号。此外，生化分级分析表明，Bmglobin和Scglobin都定位于细胞质中。这些结果表明，每一个鳞翅目珠蛋白在其表达的组织中具有不同的作用，并且昆虫珠蛋白的功能比以前认为的更加多样化。
We describe the characterization of hemoglobin-like genes from two lepidopteran insects, Bombyx mori (Bmglobin) and Samia cynthia ricini (Scglobin). Bmglobin and Scglobin are predicted to be intracellular proteins and contain amino acids required for heme and oxygen binding. Expression profiles of two lepidopteran globins, especially Bmglobin, were different from that of other insect globins. Although other insect globins are mainly associated with the tracheal system, Bmglobin was expressed almost exclusively in the Malpighian tubules, and the strongest signal for Scglobin was detected in the fat body. Furthermore, biochemical fractionation analysis revealed that both Bmglobin and Scglobin were localized in the cytoplasm. These results suggest that each lepidopteran globin has a distinct role in the tissues in which it is expressed and that the functions of insect globins are more divergent than previously thought.