Protocatechuic acid improved growth performance, meat quality, and intestinal health of Chinese yellow-feathered broilers
Protocatechuic acid improved growth performance, meat quality, and intestinal health of Chinese yellow-feathered broilers
复制标题
原儿茶酸改善黄羽肉鸡的生长性能、肉质和肠道健康
DOI：
10.3382/ps/pez124
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发表时间：
2019-08-01
期刊：
影响因子：
4.4
通讯作者：
Li, Weifen
中科院分区：
文献类型：
--
作者：
Wang, Yibing;Wang, Yuanyuan;Li, Weifen
摘要本试验旨在研究原儿茶酸（PCA）对中国黄羽肉鸡生长性能、肉品质和肠道健康的影响。结果表明，在基础饲粮中添加300、600 mg/kg PCA和200 mg/kg enramycin，可显著提高肉鸡体重、活重和胴体重，降低料重比；PCA通过降低剪切力，提高屠宰后24 h的a*（相对红度）和b*（相对黄度）来改善肉质。300 mg/kg PCA显著降低了血浆碱性磷酸酶和二胺氧化酶活性；PCA还能显著提高肝脏总抗氧化能力，降低丙二醛含量和黄嘌呤氧化酶活性。同时提高了空肠黏膜总超氧化物歧化酶、谷胱甘肽s-转移酶和谷胱甘肽过氧化物酶的活性。300 mg/kg PCA饲粮组空肠黏膜和血浆中白细胞介素-10和转化生长因子-β显著升高，白细胞介素-2和干扰素-γ显著降低。此外，凋亡相关基因在肝脏中相对表达减少，而肠屏障相关基因和免疫相关基因在空肠中相对表达增加。300 mg/kg PCA处理显著改变了肉鸡盲肠菌群α-多样性和结构，增加了厚壁菌门和放线菌门的相对丰度，降低了拟杆菌门和变形菌门的相对丰度。由此可见，主成分分析提高了肉仔鸡的饲料效率、生长性能、肉品质和抗氧化能力。此外，还能提高黄羽肉鸡肠道免疫功能，改善肠道菌群结构，有利于改善肠道健康。
ABSTRACT The aim of this study was to investigate the effect of protocatechuic acid (PCA) on the growth performance, meat quality, and intestinal health of Chinese yellow-feathered broilers. Growing broilers were fed the basal diet or diets supplemented with 300 or 600 mg/kg PCA, or 200 mg/kg enramycin for 52 D. We found that addition of 300 mg/kg PCA significantly increased body weight, live weight, and carcass weight and decreased the feed to gain ratio of broilers; PCA improved meat quality through reducing shear force, and increasing a* (relative redness) and decreasing b* (relative yellowness) at 24 h after slaughter. The activities of alkaline phosphatase and diamine oxidase in plasma were significantly decreased by administration of 300 mg/kg PCA; PCA also significantly increased total antioxidant capability and decreased malondialdehyde content and activity of xanthine oxidase in liver. Meanwhile, it enhanced activities of total superoxide dismutase, glutathione s-transferase, and glutathione peroxidase in the jejunal mucosa. Interleukin-10 and transforming growth factor-β were significantly increased in jejunal mucosa and plasma of 300 mg/kg PCA diet group, whereas interluekin-2 and interferon-γ dropped dramatically. Moreover, relative expression of apoptosis-related genes decreased in liver, whereas that of intestinal barrier-related and immunity-related genes increased in jejunum. Furthermore, 300 mg/kg PCA treatment significantly changed α-diversity and structure of the cecal microflora in broilers, with increasing relative abundance of Firmicutes and Actinobacteria while reducing Bacteroidetes and Proteobacteria. These results indicated that PCA improved the feed efficiency, growth performance, meat quality of broilers, and antioxidant capacity. It also enhanced intestinal immune function and improved the structure of intestinal flora to favor improved intestinal health in Chinese yellow-feathered broilers.