Open-tubular electrochromatographic characterization of synthetic peptides.
Open-tubular electrochromatographic characterization of synthetic peptides.
复制标题
合成肽的开管电色谱表征。
DOI：
10.1002/elps.200405893
复制
发表时间：
2004
期刊：
影响因子：
2.9
通讯作者：
Hearn,MiltonTW
中科院分区：
文献类型：
--
作者：
Pesek,JosephJ;Matyska,MariaT;Dawson,GBrent;Chen,JennyI-Chen;Boysen,ReinhardI;Hearn,MiltonTW
基于共同的结构特征，一系列肽的开管电色谱（OT-CEC）迁移行为已使用两种不同类型的化学修饰蚀刻毛细管进行了表征。固定在毛细管内表面上的有机部分分别是叔丁基苯基和胆固醇-10-十一烯酸酯。已经在几个pH值和不同浓度的甲醇作为缓冲液电解质组合物的有机溶剂改性剂下研究了这组肽的结构迁移行为。通过比较肽的结构特性，如其氨基酸序列、电荷质量比和固有疏水性与其迁移行为，确定了电泳和色谱迁移率对总迁移时间、洗脱顺序和选择性的相对贡献。此外，实验数据提供了重要的洞察程序，可用于通过改变电解质缓冲液的组成以及键合有机部分的增溶特性来调节OT-CEC中肽的分离。
The open‐tubular electrochromatographic (OT‐CEC) migration behavior of a series of peptides, based on a common structural feature, has been characterized using two different types of chemically modified etched capillaries. The organic moieties immobilized onto the capillary inner surface weren‐butylphenyl and cholesterol‐10‐undecenaoate, respectively. The structure‐migration behavior of this set of peptides has been studied at several pH values and with methanol at different concentrations as an organic solvent modifier of the buffer electrolyte composition. By comparing the structural properties of the peptides, such as their amino acid sequences, charge‐to‐mass ratios and intrinsic hydrophobicities to their migrational behavior, the relative contribution of electrophoretic and chromatographic mobility to the overall migration times, elution order, and selectivity has been determined. Moreover, the experimental data provide important insight into procedures that can be used to modulate the separation of peptides in OT‐CEC through variation of the composition of the electrolyte buffer as well asviathe properties of the bonded organic moiety.