Haemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response
Haemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response
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DOI：
10.1051/vetres:2006022
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发表时间：
2006-07-01
影响因子：
4.4
通讯作者：
Jacquiet, Philippe
中科院分区：
文献类型：
--
作者：
Lacroux, Caroline;Nguyen, Thi Hai Chi;Jacquiet, Philippe
选择抗性动物和宿主免疫已被提议作为控制胃肠道线虫寄生虫的替代方法。然而，需要更好地了解这些寄生虫的保护性免疫机制，以制定最佳策略。在本研究中，将3月龄INRA 401羔羊（n = 81）分为3组：未感染对照组，用10 000只捻转血矛线虫L-3攻毒1次（初次感染动物）或2次（既往感染动物）。在攻击后3、7、15和28天依次处死未感染的对照和攻击动物。在这两个挑战组，一个明确的Th-2导向的免疫反应，观察到在皱胃淋巴结和粘膜。IL-4和IL-13 mRNA的过度表达，嗜酸性粒细胞，肥大细胞和球白细胞的募集和特定的全身IgG和粘膜伊加的生产在以前感染的动物比在初次感染的。在感染后28天，在激发组之间未观察到这些反应强度之间的差异。既往感染和初次感染羔羊的蠕虫建立率相似。然而，在先前感染的绵羊中观察到蠕虫发育、雌性生殖力和粪便卵产量的减少。我们的结论是H. INRA 401羔羊感染捻转体诱导明确的Th-2免疫应答，导致虫卵产生的调节，而不影响其建立。
Selection of resistant animals and host immunization have been proposed as alternative methods for the control of gastrointestinal nematode parasites. However, a better knowledge of the mechanisms involved in protective immunity against these parasites is required for the development of optimal strategies. In this study, 3 month old INRA 401 lambs (n = 81) were allocated into three groups: uninfected control, challenged either once (primary-infected animals) or twice (previously-infected animals) with 10 000 Haemonchus contortus L-3. Uninfected control and challenged animals were sequentially sacrificed at 3, 7, 15 and 28 days post challenge. In both challenged groups, a clear Th-2-oriented immune response was observed in the abomasal lymph node and mucosa. IL-4 and IL-13 mRNA over-expression, recruitment of eosinophils, mast cells and globule leukocytes and production of specific systemic IgG and mucosal IgA were observed earlier in previously-infected animals than in primary-infected ones. At 28 days post infection, no differences between intensities of these responses were observed between the challenged groups. Worm establishment rates were similar in previously-infected and primary-infected lambs. However, reductions of worm development, female fecundity and fecal egg output were observed in previously-infected sheep. We conclude that H. contortus infection in young INRA 401 lambs induced an unequivocal Th-2 immune response resulting in the regulation of worm egg production without affecting their establishment.