Antitumour activities of alginate-derived oligosaccharides and their sulphated substitution derivatives
Antitumour activities of alginate-derived oligosaccharides and their sulphated substitution derivatives
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DOI：
10.1080/09670260310001636695
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发表时间：
2004-02-01
影响因子：
2.4
通讯作者：
Guan, HS
中科院分区：
文献类型：
--
作者：
Hu, XK;Jiang, XL;Guan, HS
海藻酸裂解酶。从海洋培养的弧菌sp. 510中分离出来，用于解聚海藻酸盐。采用甲酰胺-氯磺酸法制备了两种分子量不同的海藻酸衍生物寡糖。随后在昆明小鼠身上测定了这两种ADOs及其硫酸盐替代衍生物的抗肿瘤活性，但对tsFT210细胞没有直接的细胞毒作用。抗肿瘤效果最好的是寡糖A（分子量3798 Da，磺化度1.3），在100和50 mg kg（-1）剂量下对实体肉瘤180的肿瘤抑制率分别为70.4%和66.0%。对于ADOs及其硫酸盐衍生物，假设通过调节宿主介导的免疫防御来间接抗肿瘤作用。
Alginate lyase.. isolated from a marine culture of Vibrio sp. 510, was used to depolymerize alginate. Two alginate-derived oligosaccharides (ADOs) of different molecular weight were sulphated with the formamide-chlorosulphonic acid method. The antitumour activities of the two ADOs and their sulphated substitution derivatives that exhibited no direct cytotoxic effects on tsFT210 cells, were subsequently determined on Kunming mice. The best antitumour performer was Oligosaccharide A (molecular weight 3798 Da, sulphation degree 1.3), which exhibited 70.4 and 66.0% tumour inhibition against solid Sarcoma 180 at doses of 100 and 50 mg kg(-1), respectively. For ADOs and their sulphated derivatives, an indirect antitumour effect via modulation of the host-mediated immune defences is postulated.