Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
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DOI：
10.1007/s10096-020-03913-9
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发表时间：
2020-04-27
影响因子：
4.5
通讯作者：
Raoult, Didier
中科院分区：
文献类型：
--
作者：
La Scola, Bernard;Le Bideau, Marion;Raoult, Didier
在一项初步的临床研究中，我们观察到羟氯喹和阿奇霉素的联合用药通过缩短Covid-19患者的病毒载量持续时间而有效对抗SARS-CoV-2。最重要的是确定何时可以认为接受治疗的患者不再具有传染性。在细胞培养物中成功分离病毒与靶向E基因的定量RT-PCR的Ct值之间的相关性表明，使用我们的RT-PCR系统Ct高于33-34的患者不具有传染性，因此可以出院或对非住院患者进行严格限制。
In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33-34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.