Frequent serial fecal corticoid measures from rats reflect circadian and ovarian corticosterone rhythms
Frequent serial fecal corticoid measures from rats reflect circadian and ovarian corticosterone rhythms
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DOI：
10.1677/joe.1.05935
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
McClintock, MK
中科院分区：
文献类型：
--
作者：
Cavigellil, SA;Monfort, SL;McClintock, MK
昼夜糖皮质激素节律提供了个人下丘脑-垂体-肾上腺轴功能的重要信息。频繁重复的血液采样可能会限制对这种节律进行的各种研究，特别是在血容量有限且易于处理的小型实验室啮齿动物中。我们开发了一种提取和测定方案，以测量从未受干扰的雄性和雌性成年Sprague-Dawley大鼠反复收集的粪便皮质酮代谢物。这种粪便测量提供了一种非侵入性方法来评估单个动物体内皮质酮随时间的变化，具有足够的时间灵敏度来量化昼夜节律的几个特征：例如，节律的最低点、高峰期和不对称（锯齿状）。雄性比雌性分泌更多的免疫反应性粪便皮质激素。在整个发情周期中，雌性在发情前（排卵前黄体生成素激增的那一天）产生的粪便皮质激素比发情期或流星期更多。这些结果为研究个体大鼠体内皮质酮昼夜节律的环境、心理和生理紊乱奠定了基础。
The circadian glucocorticoid rhythm provides important information on the functioning of the hypothalamic-pituitary-adrenal axis in individuals. Frequent repeated blood sampling can limit the kinds of studies conducted on this rhythm, particularly in small laboratory rodents that have limited blood volumes and are easily stressed by handling. We developed an extraction and assay protocol to measure fecal corticosterone metabolites in repeated samples collected from undisturbed male and female adult Sprague-Dawley rats. This fecal measure provides a non-invasive method to assess changes in corticosterone within a single animal over time, with sufficient temporal acuity to quantify several characteristics of the circadian rhythm: e.g. the nadir, acrophase, and asymmetry (saw-tooth) of the rhythm. Males excreted more immunoreactive fecal corticoids than did females. Across the estrous cycle, females produced more fecal corticoids on proestrus (the day of the preovulatory luteinizing hormone surge) than during estrus or metestrus. These results establish a baseline from which to study environmental, psychological, and physiological disturbances of the circadian corticosterone rhythm within individual rats.