FIBROBLAST GROWTH-FACTOR (FGF) LEVELS IN THE DEVELOPING RAT-BRAIN
FIBROBLAST GROWTH-FACTOR (FGF) LEVELS IN THE DEVELOPING RAT-BRAIN
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DOI：
10.1016/0165-3806(90)90240-y
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发表时间：
1990-03-01
期刊：
影响因子：
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通讯作者：
FINKLESTEIN, SP
中科院分区：
文献类型：
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作者：
CADAY, CG;KLAGSBRUN, M;FINKLESTEIN, SP
酸性和碱性成纤维细胞生长因子（FGF）是对中枢神经系统（CNS）神经胶质、内皮细胞和神经元具有强效多能营养作用的多肽。这些因子的特征在于与肝素的强结合，并且通常通过它们在体外对Balb/c 3T3细胞的促有丝分裂活性来测定。我们发现了一个标记（CA）。13倍）增加Balb/c 3T3促有丝分裂活性在发育中的大鼠脑从胚胎期到出生后第三周。在成熟的大脑中维持高水平。大部分的促有丝分裂活性从大鼠脑强烈结合肝素亲和柱，并在酸性FGF（aFGF）和碱性FGF（bFGF）的特征位置洗脱。使用特异性抗FGF血清通过免疫印迹技术确认洗脱峰中aFGF和bFGF的存在。肝素亲和高效液相色谱法（HPLC）显示，在出生后第10天和第40天之间，aFGF水平的增加比例大于bFGF。在胚胎晚期和出生后早期脑发育阶段，FGF水平的增加可能在神经胶质和毛细血管增殖以及在此期间发生的神经元生长和突触形成中发挥重要作用。aFGF与bFGF的不同积累速率表明这些因子在发育中的大脑中具有不同的生理作用。
Acidic and basic fibroblast growth factors (FGF) are polypeptides with potent multipotential trophic effects on central nervous system (CNS) glia, endothelial cells, and neurons. These factors are characterized by strong binding to heparin, and are commonly assayed by their mitogenic activity on Balb/c 3T3 cells in vitro. We found a marked (ca. 13-fold) increase in Balb/c 3T3 mitogenic activity in the developing rat brain from the embryonic stage to the third postnatal week. High levels were sustained in the mature brain. Most of the mitogenic activity from rat brain bound strongly to heparin-affinity columns, and was eluted at positions characteristic of acidic FGF (aFGF) and basic FGF (bFGF). The presence of aFGF and bFGF in eluted peaks was confirmed by immunoblotting techniques using specific anti-FGF sera. Heparin-affinity high performance liquid chromatography (HPLC) showed a proportionately greater increase in levels of aFGF than bFGF between the tenth and fortieth postnatal days. Increases in FGF levels during late embryonic and early postnatal stages of brain development may play an important role in glial and capillary proliferation, as well as in the neuronal outgrowth and synapse formation that is occurring during this time. The differential rates of accumulation of aFGF vs bFGF suggest different physiological roles for these factors in the developing brain.