Cortisol release in response to UVB exposure in Xiphophorus fish
Cortisol release in response to UVB exposure in Xiphophorus fish
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DOI：
10.1016/j.cbpc.2014.02.004
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发表时间：
2014-06-01
影响因子：
3.9
通讯作者：
Walter, Ronald B.
中科院分区：
文献类型：
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作者：
Contreras, Adam J.;Boswell, Mikki;Walter, Ronald B.
剑尾鱼由26个已知物种组成。选择物种之间的种间杂交已被用于产生研究黑色素瘤发展的实验模型。剑尾鱼黑色素瘤诱导方案利用紫外线（UVB）诱导DNA损伤和相关的下游肿瘤发生。然而，尚未评估UVB治疗对经历肿瘤诱导方案的实验动物引起的诱导应激的影响。压力是对过度或不可预测的环境刺激的适应性生理反应。鱼的应激反应可以通过测定释放到水中的皮质醇来测量。在这里，我们提出的结果，从调查的应激反应在实验治疗和UVB暴露在剑尾鱼maculatus Jp 163 B，剑尾鱼couchianus，和F-1种间杂交产生的交配X。斑叶野牡丹（X. maculatus Jp 163 B x X.）Couchianus。总的来说，UVB照射后男性和女性的皮质醇释放率没有统计学差异。在较低的UVB剂量（8和16 kJ/m2）下，X. Couchianus的DNA损伤水平比X. maculatus或F1杂种。然而，根据皮质醇释放率，没有一种鱼在UVB较低剂量（8和16 kJ/m2）下引起初级应激反应。相反，在非常高的UVB剂量（32 kJ/m2）下，X. maculatus和F-1杂种的皮质醇释放速率增加了5倍。为了确定色素沉着对UVB诱导的应激的影响，将野生型和白化病剑尾鱼暴露于UVB（32 kJ/m2）。白化病X. hellerii显示皮质醇释放增加3.7倍，而野生型X hellerii对UVB没有显示显著的皮质醇响应。总的来说，这些数据表明，在肿瘤诱导方案中经常采用的相当低的UVB剂量不会诱导剑尾鱼的主要应激反应。(C)版权所有© 2014 Elsevier Inc.
Xiphophorus fishes are comprised of 26 known species. Interspecies hybridization between select species has been utilized to produce experimental models to study melanoma development. Xiphophorus melanoma induction protocols utilize ultraviolet light (UVB) to induce DNA damage and associated downstream tumorigenesis. However, the impact of induced stress caused by the UVB treatment of the experimental animals undergoing tumor induction protocols has not been assessed. Stress is an adaptive physiological response to excessive or unpredictable environmental stimuli. The stress response in fishes may be measured by an assay of cortisol released into the water. Here, we present results from investigations of stress response during an experimental treatment and UVB exposure in Xiphophorus maculatus Jp 163 B, Xiphophorus couchianus, and F-1 interspecies hybrids produced from the mating X. maculatus Jp 163 B x X. couchianus. Overall, cortisol release rates for males and females after UVB exposure showed no statistical differences. At lower UVB doses (8 and 16 kJ/m(2)), X. couchianus exhibited 2 fold higher levels of DNA damage then either X. maculatus or the F1 hybrid. However, based on the cortisol release rates, none of the fish types tested induced a primary stress response at the UVB lower doses (8 and 16 kJ/m(2)). In contrast, at a very high UVB dose (32 kJ/m2) both X. maculatus and the F-1 hybrid showed a 5 fold increase in the cortisol release rate. To determine the effect of pigmentation on UVB induced stress, wild type and albino Xiphophorus hellerii were exposed to UVB (32 kJ/m(2)). Albino X. hellerii exhibited 3.7 fold increase in the cortisol release while wild type X hellerii did not exhibit a significant cortisol response to UVB. Overall, the data suggest the rather low UVB doses often employed in tumor induction protocols do not induce a primary stress response in Xiphophorus fishes. (C) 2014 Elsevier Inc All rights reserved.