Ultrastructure of E-PTA stained synaptic ribbons in the chick retina.
Ultrastructure of E-PTA stained synaptic ribbons in the chick retina.
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E-PTA 染色的小鸡视网膜突触带的超微结构。
DOI：
10.1002/neu.480080107
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
L. Boykins
中科院分区：
文献类型：
--
作者：
B. Mclaughlin;L. Boykins
有人认为，突触带在功能上等同于传统突触中的致密突起（Gray和Pease，1971），并且它的功能是通过连接突触带与囊泡的类似突触的条纹将突触囊泡以有序的方式运送到突触前膜（Bunt，1971）。本文用Bloom和Aghajanian（1966）的乙醇磷钨酸（E-PTA）技术研究了鸡视网膜感光细胞突触带的丝状连接，并进一步描述了可能与其功能有关的这些带丝的超微结构特征（拉德，70%）、2%多聚甲醛、0.5%丙烯醛、0.5%二甲亚砜的0.08 M磷酸盐缓冲液（Skoff和Hamburger，1974）。切下颞下象限并在4 ℃下在相同的固定剂中保存18小时，然后用手切成小块，脱水并在1%PTA的无水乙醇溶液中搅拌染色1小时，每25 ml染色溶液含有3滴95%乙醇（Bloom和Aghajanian，1966）。然后将组织在无水乙醇中脱水，在环氧丙烷中冲洗，并包埋在Epon-Araldite中。在金刚石刀上切割薄切片，并在菲利普斯EM 301中进行检查，无需进一步染色。
It has been suggested that the synaptic ribbon is functionally equivalent to the dense projections in conventional synapses (Gray and Pease, 1971) and that it functions to convey the synaptic vesicles in an orderly fashion to the presynaptic membrane by way of filamentous-like striations connecting the ribbon with the vesicles (Bunt, 1971). This report examines the filamentous connections of photoreceptor synaptic ribbons in the chick retina using the ethanolic phosphotungstic acid (E-PTA) technique of Bloom and Aghajanian (1966) and describes further ultrastructural features of these ribbon filaments which may be related to their proposed function.Chick retinas from 19-day old embryos and 2-day old hatchlings were fixed by ocular perfusion with 2% glutaraldehyde (Ladd, 70%), 2% paraformaldehyde, 0.5% acrolein, 0.5% dimethylsulfoxide in 0.08 M phosphate buffer (Skoff and Hamburger, 1974). The lower temporal quadrant was dissected out and stored in the same fixative for 18 hr at 4 C and then hand dissected into smaller pieces, dehydrated and stained 1 hr with agitation in 1% PTA in absolute ethanol, containing three drops of 95% ethanol per 25 ml staining solution (Bloom and Aghajanian, 1966). The tissue was then dehydrated in absolute ethanol, rinsed in propylene oxide, and embedded in Epon-Araldite. Thin sections were cut on a diamond knife and examined without further staining in a Phillips EM 301.