Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH).
Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH).
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DOI：
10.1007/978-1-0716-2128-8_19
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发表时间：
2022
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肝细胞脂毒性是非酒精性脂肪性肝炎（NASH）的标志，脂质诱导的肝损伤部分是通过内质网（ER）应激的激活而发生的。因此，未折叠蛋白反应（UPR）被启动，由三个关键的内质网跨膜蛋白驱动，导致下游反应是动态和相互关联的。因此，研究内质网应激在NASH中的复杂作用需要仔细研究这些通路。在此，我们描述了小鼠肝细胞脂毒性内质网应激评估的不同机制和体外试验。
Hepatocyte lipotoxicity is a hallmark of nonalcoholic steatohepatitis (NASH), and lipid induced liver injury occurs, in part, via activation of endoplasmic reticulum (ER) stress. Consequently, the unfolded protein response (UPR) is initiated, driven by three key ER transmembrane proteins, resulting in downstream responses that are dynamic and interconnected. Thus, careful interrogation of these pathways is required to investigate the complex role of ER stress in NASH. Herein, we describe different mechanisms of and in vitro assays for assessment of lipotoxic ER stress in mouse hepatocytes.