Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients
Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients
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DOI：
10.1590/s0102-86502013000800001
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发表时间：
2013-08-01
影响因子：
1.1
通讯作者：
Ferreira, Lydia Masako
中科院分区：
文献类型：
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作者：
Gragnani, Alfredo;Müller, Bruno Rafael;Ferreira, Lydia Masako
目得：为探讨KGF、TNF-α和IL-1 β在烧伤患者皮肤成纤维细胞和角质形成细胞中的基因表达情况，选取3例大面积烧伤患者、3例小面积烧伤患者和2例正常对照。通过酶促方法启动细胞培养。结果：与正常人相比，大面积烧伤后成纤维细胞中KGF的平均表达量增加220倍，小面积烧伤后增加33.33倍，角质形成细胞中KGF的平均表达量增加11.2倍，小面积烧伤后增加3.45倍（p < 0.01）。
PURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients.METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta.RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p