Morphologic alterations in ovine placenta and fetal liver following induced severe placental insufficiency.
Morphologic alterations in ovine placenta and fetal liver following induced severe placental insufficiency.
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诱发严重胎盘功能不全后绵羊胎盘和胎儿肝脏的形态学改变。
DOI：
10.1016/j.jsgi.2004.06.010
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Brace,RobertA
中科院分区：
文献类型：
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作者：
Cheung,CeciliaY;Bogic,Ljubica;Gagnon,Robert;Harding,Richard;Brace,RobertA
目的：微球脐-胎盘栓塞已被用作胎盘功能不全和宫内生长受限（IUGR）的模型。然而，栓塞对胎盘结构和器官形态学的IUGR胎儿的影响是相对未知的。在这项研究中，使用羊胎儿，我们确定了胎盘内的微球的位置和分布，并探讨了胎盘栓塞引起的胎盘和胎儿器官形态学变化的程度。我们假设，微球管理到脐循环超过4天将导致胎盘损伤，而没有显着的形态学改变，胎儿肾脏或肝脏。方法：11只妊娠绵羊在118 ± 1（SE）天的妊娠进行了研究。在6个胎儿中，通过将直径为15 μm的微球连续4天注射到胎儿脐动脉近端的降主动脉中诱导栓塞。五个胎儿作为时间controls.Results：在栓塞胎儿，微球被检测到在胎盘中嵌入胎儿细胞滋养层的母体实质相邻的绒毛细胞滋养层。胎儿细胞滋养层细胞表现正常，除了胎儿和母体细胞层之间失去明显的分离。在毛细血管内的胎膜中也检测到微球。栓塞胎仔的体重低于对照组，体重标准化的肝脏重量也低于对照组，但肾脏重量不低于对照组。在栓塞胎儿的肝脏中，造血细胞簇的数量显着减少，而胎儿肾脏出现normal.Conclusions：我们得出结论，经过5天的脐胎盘栓塞，微球集中在胎儿绒毛近端的顶端母胎界面和膜。栓塞的胎盘有明显的形态学变化，胎盘无明显的大体损伤。与栓塞相关的胎儿肝脏重量和肝脏髓外造血细胞丰度的降低可能使胎儿易于发生出生后持续存在的肝功能改变。
Objectives:Umbilical-placental embolization with microspheres has been used as a model of placental insufficiency and intrauterine growth restriction (IUGR). However, the effects of embolization on placental structure and organ morphology of the resulting IUGR fetus are relatively unexplored. In this study using ovine fetuses, we determined the location and distribution of microspheres within the placenta and explored the extent of placental and fetal organ morphologic changes induced by placental embolization. We hypothesized that microspheres administered into the umbilical circulation over 4 days would cause placental damage without significant morphologic alterations in fetal kidney or liver.Methods:Eleven pregnant sheep at 118 ± 1 (SE) days' gestation were studied. In six fetuses, embolization was induced by injection of 15-μm diameter microspheres on 4 successive days into the fetal descending aorta proximal to the umbilical arteries. Five fetuses served as time controls.Results:In embolized fetuses, microspheres were detected in the placenta embedded in the fetal cytotrophoblastic layer on maternal parenchyma adjacent to villous cytotrophoblasts. Fetal cytotrophoblasts appeared normal except for loss of distinct separation between fetal and maternal cell layers. Microspheres were also detected in the fetal membranes within capillaries. The body weights of embolized fetuses were lower than controls, as were the body weight-normalized liver but not kidney weights. In the liver of the embolized fetuses, the number of hematopoietic cell clusters was markedly reduced, whereas the fetal kidneys appeared normal.Conclusions:We conclude that after 5 days of umbilical-placental embolization, microspheres were concentrated at the fetal villi proximal to the apical maternal-fetal interface and in the membranes. There were noticeable morphologic changes in the embolized placentas, with no apparent gross damage to the placenta. The reduction in fetal liver weight and liver extramedullary hematopoietic cell abundance associated with embolization may predispose the fetus to alterations in liver function that could persist after birth.