PSEUDOMONAS-AERUGINOSA INVASION OF AND MULTIPLICATION WITHIN CORNEAL EPITHELIAL-CELLS IN-VITRO
PSEUDOMONAS-AERUGINOSA INVASION OF AND MULTIPLICATION WITHIN CORNEAL EPITHELIAL-CELLS IN-VITRO
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DOI：
10.1128/iai.63.10.4072-4077.1995
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发表时间：
1995-10-01
影响因子：
3.1
通讯作者：
PIER, GB
中科院分区：
文献类型：
--
作者：
FLEISZIG, SMJ;ZAIDI, TS;PIER, GB
铜绿假单胞菌通常被认为是一种胞外病原体。我们先前已经证明，在小鼠感染性角膜炎期间，铜绿假单胞菌能够入侵角膜细胞，通过测定庆大霉素的存在来确定细胞内的存活情况。在体外，发现铜绿假单胞菌进入以下细胞：通过冲洗去除的人角膜细胞；大鼠、小鼠和兔的角膜上皮细胞；以及大鼠和兔眼的原代培养的角膜上皮细胞。侵袭程度与粘附菌或伴生菌的水平有关。一般来说，培养的上皮细胞比原位检测的细胞侵袭效率更高。当在4℃下进行检测时，没有发生侵袭，细胞松弛素D而不是秋水仙素抑制了细菌的侵袭，这表明细菌进入是一个依赖于肌动蛋白微丝而不是微管的内吞过程。侵袭培养的角膜上皮细胞的细菌被发现在细胞内繁殖。在感染性角膜炎期间，铜绿假单胞菌在角膜上皮细胞内侵袭和繁殖的能力可能有助于该细菌的毒力，因为细胞内细菌可以逃避宿主免疫效应器和常用的治疗感染的抗生素。
Pseudomonas aeruginosa is usually considered an extracellular pathogen. Using assays to determine intracellular survival in the presence of gentamicin, we have previously demonstrated that P. aeruginosa is able to invade corneal cells during infectious keratitis in mice. In vitro, P. aeruginosa was found to enter the following cells: human corneal cells removed by irrigation; epithelial cells in the cornea of rats, mice, and rabbits; and primary corneal epithelial cells cultured from rat and rabbit eyes. The level of invasion was related to the level of adherent or associated bacteria. In general, invasion was more efficient with cultured epithelial cells than with cells tested in situ. Invasion did not occur when assays were performed at 4 degrees C. Cytochalasin D brit not colchicine inhibited bacterial invasion, suggesting that bacterial entry was an endocytic process dependent on actin microfilaments but not microtubules. Bacteria that invaded cultured corneal epithelial cells were found to multiply within cells. The ability of P. aeruginosa to invade and multiply within corneal epithelial cells may contribute to the virulence of this organism during infectious keratitis, since intracellular bacteria can evade host immune effecters and antibiotics commonly used to treat infection.