Effects of short-term cold acclimation on FoF1-ATPase activity in skeletal muscle of red seabream Pagrus major (Temminck & Schlegel).
Effects of short-term cold acclimation on FoF1-ATPase activity in skeletal muscle of red seabream Pagrus major (Temminck & Schlegel).
复制标题
短期冷驯化对真鲷骨骼肌FoF1-ATP酶活性的影响
DOI：
10.1111/are.12132
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Watabe S.
中科院分区：
文献类型：
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作者：
Kaneko G;Sawada A;Ushio H;Watabe S.
适应寒冷温度的鱼会表现出许多生理生化变化，以补偿代谢酶活性的下降。冷驯化期间的主要变化之一是骨骼肌中线粒体的增加（Guderley 2004）。例如，在鲫鱼 Carassius carassius (Linnaeus) 中，适应 2°C 的鱼的肌肉纤维中线粒体体积密度比适应 28°C 的鱼高两到四倍（Johnston & Maitland 1980）。后来的研究表明，在几种鱼类的冷驯化过程中，线粒体 ATP 合酶 (FoF1-ATPase) 的数量或活性随之增加，包括鲤鱼 Cyprinus carpio (Linnaeus)(Itoi, Kinoshita, Kikuchi & Watabe 2003)、真鲷 Pagrus Major (Temminck & Schlegel) 和日本比目鱼 Paralichthys olivaceus （Temminck 和 Schlegel）（Itoi、Ikeguchi、Kaneniwa、Kuwahara、Ohara、Ishida、Yamashita 和 Watabe 2007）。这些变化将促进骨骼肌在低温下产生 ATP，从而提高游泳性能。另一方面，众所周知，冷驯化会延长鱼类的前僵直期。适应 10°C 5 周的鲤鱼比适应 30°C 的鲤鱼有更长的预严酷期（Watabe、Hwang、Ushio 和 Hashimoto 1990；Hwang、Ushio、Watabe、Iwamoto 和 Hashimoto 1991）。此外，冬季捕获的红鲷也表现出
Fish acclimated to cold temperature show a number of physiological and biochemical changes to compensate for the decrease in the activity of metabolic enzymes. One of the major changes during cold acclimation is an increase in mitochondria in skeletal muscle (Guderley 2004). In crucian carp Carassius carassius (Linnaeus), for example, mitochondrial volume density in muscle fibres was two to fourfolds higher in fish acclimated to 2 C than those to 28 C (Johnston & Maitland 1980). Later studies demonstrated that mitochondrial ATP synthase (FoF1-ATPase) shows a concomitant increase in its amount or activity during cold acclimation in several fish species including carp Cyprinus carpio (Linnaeus)(Itoi, Kinoshita, Kikuchi & Watabe 2003), red seabream Pagrus major (Temminck & Schlegel) and Japanese flounder Paralichthys olivaceus (Temminck & Schlegel)(Itoi, Ikeguchi, Kaneniwa, Kuwahara, Ohara, Ishida, Yamashita & Watabe 2007). These changes would facilitate ATP production in skeletal muscle at cold temperatures, resulting in an improvement of swimming performance.On the other hand, cold acclimation is known to increase the pre-rigour period of fish. Carp acclimated to 10 C for 5 weeks had a longer pre-rigour period than those to 30 C (Watabe, Hwang, Ushio & Hashimoto 1990; Hwang, Ushio, Watabe, Iwamoto & Hashimoto 1991). In addition, red seabream caught in winter also exhibited a