Multiple glutathione S-transferase genes: identification and expression in oriental fruit fly, Bactrocera dorsalis
Multiple glutathione S-transferase genes: identification and expression in oriental fruit fly, Bactrocera dorsalis
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DOI：
10.1002/ps.3558
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发表时间：
2014-02-01
影响因子：
4.1
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
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作者：
Hu, Fei;Dou, Wei;Wang, Jin-Jun
背景东方实蝇（Bactrocera dorsalis（Hendel））广泛分布于亚太地区，是热带和亚热带果蔬作物的重要害虫。本研究从B.结果系统发育分析显示，16个GST基因分别属于5个不同的胞质类型，其中δ 4个，β 8个，ω 2个，θ 1个，ζ 1个。其余GST（BdGSTu 1）未分类。RT-qPCR结果显示，5个GST基因在幼虫期的相对表达量均显著高于成虫期。组织特异性表达分析发现，BdGSTe 3、BdGSTe 9和BdGSTd 5在中肠中表达量较高，BdGSTe 4、BdGSTe 6、BdGSTd 6和BdGSTz 2在脂肪体中表达量较高，6种GST在马氏管中表达量较高。RT-qPCR结果表明，马拉硫磷暴露后不同时间和不同剂量的处理均使9种GST基因表达量增加，而BdGSTe 4、BdGSTe 9和BdGSTt 1基因表达量则随氯氰菊酯暴露量的增加而增加。dorsalis可能会增加该物种对其他杀虫剂和异生物质的解毒能力。(c)2013年化学工业协会
BackgroundThe oriental fruit fly, Bactrocera dorsalis (Hendel), is widely distributed in Asia-Pacific regions, where it is a serious pest of a wide range of tropical and subtropical fruit and vegetable crops. In this study, 17 cDNA encoding glutathione S-transferases (GSTs) in B. dorsalis were sequenced and characterised.ResultsPhylogenetic analysis revealed that 16 GSTs belonged to five different cytosolic classes, including four in delta, eight in epsilon, two in omega, one in theta, and one in zeta. The remaining GST (BdGSTu1) was unclassified. RT-qPCR assay showed that the relative expression levels of five GST genes were significantly higher in larval stages than in adulthood. Tissue-specific expression analysis found that BdGSTe3, BdGSTe9 and BdGSTd5 were expressed highly in the midgut, BdGSTe4, BdGSTe6, BdGSTd6 and BdGSTz2 were higher in the fat body, and six GSTs were higher in Malpighian tubules. RT-qPCR confirmed that the expressions of nine GST genes were increased by malathion exposure at various times and doses, while BdGSTe4, BdGSTe9 and BdGSTt1 were increased by -cypermethrin exposure.ConclusionThe increases in GST gene expression levels after malathion and -cypermethrin exposure in B. dorsalis might increase the ability of this species to detoxify other insecticides and xenobiotics. (c) 2013 Society of Chemical Industry