Detection and molecular characterization of two rare G8P[14] and G3P[3] rotavirus strains collected from children with acute gastroenteritis in Japan
Detection and molecular characterization of two rare G8P[14] and G3P[3] rotavirus strains collected from children with acute gastroenteritis in Japan
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DOI：
10.1016/j.meegid.2018.04.011
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发表时间：
2018-08-01
影响因子：
3.2
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Okitsu, Shoko;Hikita, Toshiyuki;Ushijima, Hiroshi
本研究描述了2014年从日本急性胃肠炎儿童中收集的两种罕见G8 P [14]和G3 P [3]轮状病毒株的检测和分子特征。在247株轮状病毒中，检出1株G8 P [14]（12，597株）和1株G3 P [3]（12，638株）轮状病毒。对这两种轮状病毒株（RVA/Human-wt/JPN/12597/2014/G8 P [14]和RVA/Human-wt/JPN/12638/2014/G3 P [3]）的11个基因片段的基因型进行了表征。12，597株的基因型星座为G8 P [14]-I2-R2-C2-M2-A3-N2-T9-E2-H3，该株具有罕见的NSP 3基因T9基因型，与VP 7基因G8基因型结合在一起未见报道。分子特征和系统发育分析表明，菌株12，597具有来自动物源如牛、鹿、狗和猫的轮状病毒的共有G8 P骨架[14]。菌株12，638的基因型星座被鉴定为G3-P[3]-13-R3-C3-M3-A9-N2-T3-E3-H6。12，638株的VP 7和VP 4基因型与Cat 97-like株相似，而VP 1、VP 2和VP 3基因型与Au-1-like株密切相关。有趣的是，NSP 1至NSP 3基因与蝙蝠轮状病毒（RVA/Bat-wt/ZMB/LUS 12 -14/2012/G3 P [3]毒株）的基因具有最高的同一性。这些结果表明，菌株12，638是Au-1样菌株、Cat 97样菌株和蝙蝠菌株中的基因型内回归菌株。有趣的是，菌株12，597和12，638具有相同的NSP 2基因的N2基因型。本研究的结果支持了种间传播和多重重配事件在人类轮状病毒遗传多样性产生中的可能作用。
This study describes the detection and molecular characterization of two rare G8P[14] and G3P[3] rotavirus strains, which were collected from children with acute gastroenteritis in 2014 in Japan. Among 247 rotaviruses, one G8P[14] (strain 12,597) and one G3P[3] (strain 12,638) rotaviruses were detected. The genotypes of 11 gene segments of these two rotavirus strains (RVA/Human-wt/JPN/12597/2014/G8P [14] and RVA/Human-wt/JPN/12638/2014/G3P [3]) were characterized. The genotype constellation of strain 12,597 was assigned to G8P[14]-I2-R2-C2-M2-A3-N2-T9-E2-H3, and this strain possessed a rare T9 genotype of NSP3 gene which has never been reported previously in combination with G8 genotype of VP7 gene. Molecular characterization and phylogenetic analysis suggested that the strain 12,597 had the consensus G8P[14] backbone that originated from the rotaviruses of animal origins such as cows, deer, dogs, and cats. The genotype constellation of strain 12,638 was identified as G3-P[3]-13-R3-C3-M3-A9-N2-T3-E3-H6. The VP7 and VP4 genotypes of strain 12,638 was similar to those of the Cat97-like strains, but the VP1, VP2, and VP3 were closely related to those of the AU-1-like strain. Interestingly, the NSP1 to NSP3 genes shared highest identities with those of a bat rotavirus (RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3] strain). These findings indicated that the strain 12,638 was an intra-genotype reassortant strain among the AU-1-like strains, the Cat97-like strains and the bat strain. Interestingly, the strains 12,597 and 12,638 possessed the same N2 genotype of NSP2 gene. The results of this study support the possible roles of interspecies transmission and multiple reassortment events for generating the genetic diversity of rotavirus in human.