Ultrasound irradiation promoted lipase-catalyzed synthesis of flavonoid esters with unsaturated fatty acids
Ultrasound irradiation promoted lipase-catalyzed synthesis of flavonoid esters with unsaturated fatty acids
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超声辐照促进脂肪酶催化不饱和脂肪酸黄酮酯的合成
DOI：
10.1016/j.molcatb.2013.05.028
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发表时间：
2013-11-01
影响因子：
--
通讯作者：
Wan, Chu-Yun
中科院分区：
文献类型：
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作者：
Zheng, Ming-Ming;Wang, Lian;Wan, Chu-Yun
研究了超声预处理条件下Novozym 435催化芦丁和柚皮苷与不同游离不饱和脂肪酸的酯化反应，制备相应的黄酮酯。确定了超声频率为25 kHz、功率为200 W、功率为150 W、超声时间为1 h时，芦丁和柚皮苷的酯化率和脂肪酶活力均较好。随后考察了底物浓度和摩尔比的影响。结果表明，在丙酮和2-甲基-2-丁醇为溶剂、黄酮浓度为40 mM、黄酮与脂肪酸的摩尔比为1：4、温度为50 ℃、芦丁为溶剂、柚皮苷为溶剂的条件下，黄酮酯的转化率在83.2%以上，反应时间较短（48-72 h）。在最佳工艺条件下，超声波预处理比机械搅拌法缩短了总酯化反应时间24 h，且不影响酶活。(C)2013爱思唯尔有限公司版权所有。
This study was focused on the enzymatic esterification of rutin and naringin with different free unsaturated fatty acids to produce the corresponding flavonoid esters catalyzed by Novozym 435 under ultrasound pretreatment. An ultrasonic frequency of 25 kHz, power of 200W for rutin and 150W for naringin and time of 1 h was determined to guarantee satisfactory degree of esterification and lipase activity. The influence of substrates concentration and molar ratio was investigated subsequently. The optimum production was achieved in component solvent of acetone and 2-methyl-2-butanol for rutin and pure 2-methyl-2-butanol for naringin at 50 degrees C with flavonoid concentration of 40 mM and flavonoid to fatty acid molar ratio of 1:4, resulting in a flavonoid esters conversion of above 83.2% in relative short reaction time (48-72 h). In optimum conditions, the overall esterification reaction time using the ultrasonic pretreatment process was 24h shorter than that of mechanical stirring process without damaging the lipase activity. (C) 2013 Elsevier B.V. All rights reserved.