Rapid fabrication of precise high-throughput filters from membrane protein nanosheets
Rapid fabrication of precise high-throughput filters from membrane protein nanosheets
复制标题
DOI：
10.1038/s41563-019-0577-z
复制
发表时间：
2020-01
期刊：
影响因子：
41.2
通讯作者：
Yu-Ming Tu;Woochul Song;Tingwei Ren;Yue-xiao Shen;Ratul Chowdhury;P. Rajapaksha;Tyler E. Culp;Laxmicharan Samineni;Chao Lang;Alina Thokkadam;Drew Carson;Yuxuan Dai;A. Mukthar;Miao Zhang;A. Parshin;Janna N. Sloand;Scott H. Medina;M. Grzelakowski;Dibakar Bhattacharya;W. Phillip;E. Gomez;R. Hickey;Yi-Min Wei;Manish Kumar
中科院分区：
文献类型：
--
作者：
Yu-Ming Tu;Woochul Song;Tingwei Ren;Yue-xiao Shen;Ratul Chowdhury;P. Rajapaksha;Tyler E. Culp;Laxmicharan Samineni;Chao Lang;Alina Thokkadam;Drew Carson;Yuxuan Dai;A. Mukthar;Miao Zhang;A. Parshin;Janna N. Sloand;Scott H. Medina;M. Grzelakowski;Dibakar Bhattacharya;W. Phillip;E. Gomez;R. Hickey;Yi-Min Wei;Manish Kumar
生物膜是分离的理想选择，因为它们具有高渗透性，同时由于存在专门的膜蛋白（MP）通道而保持高溶质选择性。然而，将MPs成功整合到人造膜中仍然是一个重大挑战。在这里，我们展示了一种两小时的有机溶剂方法来开发嵌段共聚物（bcp）中高度堆积的成孔MPs的二维晶体和纳米片。然后，我们将这些混合材料集成到可扩展的MP-BCP仿生膜中。这些MP- bcp纳米片膜保持了所使用的三种孔径为0.8 nm、1.3 nm和1.5 nm的β-桶状MP通道的分子选择性。这些仿生膜的透水性比商用膜高20 - 1000倍，比具有类似分子排斥等级的最新研究膜高1.5-45倍。这种方法可以在具有挑战性的亚纳米到几纳米尺寸范围内提供高性能的替代方案。
Biological membranes are ideal for separations as they provide high permeability while maintaining high solute selectivity due to the presence of specialized membrane protein (MP) channels. However, successful integration of MPs into manufactured membranes has remained a significant challenge. Here, we demonstrate a two-hour organic solvent method to develop 2D crystals and nanosheets of highly packed pore-forming MPs in block copolymers (BCPs). We then integrate these hybrid materials into scalable MP-BCP biomimetic membranes. These MP-BCP nanosheet membranes maintain the molecular selectivity of the three types of β-barrel MP channels used, with pore sizes of 0.8 nm, 1.3 nm, and 1.5 nm. These biomimetic membranes demonstrate water permeability that is 20–1,000 times greater than that of commercial membranes and 1.5–45 times greater than that of the latest research membranes with comparable molecular exclusion ratings. This approach could provide high performance alternatives in the challenging sub-nanometre to few-nanometre size range.