Resolution of triacylglycerol positional isomers by reversed-phase high-performance liquid chromatography
Resolution of triacylglycerol positional isomers by reversed-phase high-performance liquid chromatography
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DOI：
10.1002/jssc.200401746
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发表时间：
2004-08-01
影响因子：
3.1
通讯作者：
Kuksis, A
中科院分区：
文献类型：
--
作者：
Momchilova, S;Tsuji, K;Kuksis, A
首次证明了反相高效液相色谱 (RP-HPLC) 将一些位置异构的二饱和和单不饱和三酰甘油 (TAG) 作为完整物质进行分离的能力。测试了用甲醇、乙醇、2-丙醇、1-丙醇、1-丁醇、丙酮或二氯甲烷改性的乙腈流动相在单一残留物上分离 POP-PPO、PLP-PPL、PEP-PPE 和 PDP-PPD（P - 棕榈酸、0 - 油酸、L - 亚油酸、E - 二十碳五烯酸、D - 二十二碳六烯酸残基） RP-HPLC 柱。分辨率随着酰基残基中双键数量的增加而提高。虽然 POP 和 PPO 仅部分分离，但 PDP 和 PPID 使用所有测试的流动相（含甲醇的流动相除外）完全分离。还分离了具有相同碳数 (ECN = 42) 的四个 TAG、PEP、PPE、PDP 和 PPD，在单个 RP-HPLC 柱上使用乙腈-2-丙醇 (70:30，v/v) 以 0.8 mL/min 为流动相。在所有情况下，在 1-或 3-位具有不饱和酰基残基的异构体比相应的 2-异构体被更强地保留。
The ability of reversed-phase high-performance liquid chromatography (RP-HPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethanol, 2-propanol, 1-propanol, 1-butanol, acetone, or dichloromethane were tested for the separation of POP-PPO, PLP-PPL, PEP-PPE, and PDP-PPD (P - palmitic, 0 - oleic, L - linoleic, E - eicosapentaenoic, D - docosahexaenoic acid residue) on a single RP-HPLC column. The resolution improved with increasing number of double bonds in the acyl residues. While POP and PPO were only partially resolved, PDP and PPID were fully separated with all tested mobile phases, except those containing methanol. Also separated were the four TAGs having the same equivalent carbon number (ECN = 42), PEP, PPE, PDP, and PPD, on a single RP-HPLC column with mobile phase acetonitrile-2-propanol (70:30, v/v) at 0.8 mL/min. In all cases the isomer with the unsaturated acyl residue in either 1 - or 3-position was retained more strongly than the respective 2-isomer.