Patterned Deposition of Xylan and Lignin is Independent from that of the Secondary Wall Cellulose of Arabidopsis Xylem Vessels[OPEN]
Patterned Deposition of Xylan and Lignin is Independent from that of the Secondary Wall Cellulose of Arabidopsis Xylem Vessels[OPEN]
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木聚糖和木质素的图案化沉积独立于拟南芥木质部导管的次生壁纤维素的沉积[打开]
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
T. Demura
中科院分区：
文献类型：
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作者：
Yuto Takenaka;Yoichiro Watanabe;Mathias Schuetz;Faride Unda;Joseph L. Hill;P. Phookaew;A. Yoneda;S. Mansfield;L. Samuels;M. Ohtani;T. Demura
一种新的cesa 7突变体显示，图案沉积的非纤维素次生壁聚合物，木聚糖和木质素，是不依赖于木质部导管分化过程中的纤维素图案。木质部导管细胞的次生细胞壁（SCW）提供了刚性和强度，使整个植物有效的水分传导。为了深入了解SCW沉积，我们诱变拟南芥血管相关的NAC-DOMAIN 7诱导型植物系，其中异位原生木质部导管细胞分化是同步诱导的。基于异位诱导的原生木质部导管细胞中不存在螺旋SCW模式，分离出杆状体突变体，并且成熟杆状体植物在成熟植物中表现出不规则木质部（irx）突变体表型。鉴定了杆状菌中纤维素合成酶亚单位7（CESA 7）基因中的单个核酸取代：虽然预测该突变产生C-末端截短的蛋白，但免疫印迹分析显示cesa 7 bac突变导致CESA 7蛋白的产生丧失，表明杆状菌是一种新的cesa 7功能丧失突变体。在cesa 7 bac中，尽管缺乏图案化的纤维素沉积，但其他SCW组分（如半纤维素木聚糖和酚类聚合物木质素）的螺旋图案化沉积不受影响。在另一个点突变突变体cesa 7 mur 10 -2和已建立的敲除突变体cesa 7irx 3 -4中发现了相似的表型。两者合计，我们提出，不同的SCW组件，如木聚糖和木质素的时空沉积，是不依赖于纤维素图案。
A novel cesa7 mutant revealed that patterned deposition of non-cellulosic secondary wall polymers, xylan and lignin, is not dependent on cellulose patterning during xylem vessel differentiation. The secondary cell wall (SCW) of xylem vessel cells provides rigidity and strength that enables efficient water conduction throughout the plant. To gain insight into SCW deposition, we mutagenized Arabidopsis thaliana VASCULAR-RELATED NAC-DOMAIN7-inducible plant lines, in which ectopic protoxylem vessel cell differentiation is synchronously induced. The baculites mutant was isolated based on the absence of helical SCW patterns in ectopically-induced protoxylem vessel cells, and mature baculites plants exhibited an irregular xylem (irx) mutant phenotype in mature plants. A single nucleic acid substitution in the CELLULOSE SYNTHASE SUBUNIT 7 (CESA7) gene in baculites was identified: while the mutation was predicted to produce a C-terminal truncated protein, immunoblot analysis revealed that cesa7bac mutation results in loss of production of CESA7 proteins, indicating that baculites is a novel cesa7 loss-of-function mutant. In cesa7bac, despite a lack of patterned cellulose deposition, the helically-patterned deposition of other SCW components, such as the hemicellulose xylan and the phenolic polymer lignin, was not affected. Similar phenotypes were found in another point mutation mutant cesa7mur10-2, and an established knock-out mutant, cesa7irx3-4. Taken together, we propose that the spatio-temporal deposition of different SCW components, such as xylan and lignin, is not dependent on cellulose patterning.
影响因子：
7.2
作者：
Brown, David;Wightman, Raymond;Turner, Simon
通讯作者：
Turner, Simon
影响因子：
7.2
作者：
Brown, David M.;Zhang, Zhinong;Turner, Simon R.
通讯作者：
Turner, Simon R.