Microcirculatory models of ischaemia-reperfusion in skin and striated muscle
Microcirculatory models of ischaemia-reperfusion in skin and striated muscle
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DOI：
10.1159/000179088
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Messmer, K
中科院分区：
文献类型：
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作者：
Nolte, D;Menger, MD;Messmer, K
活体显微术用于分析各种器官的微循环，如肠系膜、肠、心、肝和肺，需要麻醉动物的外显或原位可视化技术。相比之下，透明腔体的植入已经被用来观察完整的、未麻醉的动物的微循环。本文报道了两种透明腔室模型的结果：仓鼠和小鼠的皮肤折叠腔室模型。概述了腔室技术的技术发展，以及使用这些模型在横纹肌缺血再灌注中进行的各种实验研究。特别强调对用于诱导缺血和再灌注的方法的描述，即压力诱导或止血带诱导的缺血。前者可以模拟室室综合征的病理生理情况，后者可以适当模拟血管、移植和重建手术的临床情况。概述了这些微循环模型在缺血-再灌注病理生理学研究中的未来可能性，并讨论了保护缺血后组织的潜在治疗措施。
Intravital microscopy is used for analysis of the microcirculation in various organs, e.g. mesentery, intestine, heart, liver and lung, requiring either exteriorization or in situ visualization techniques in anaesthetized animals. in contrast, the implantation of transparent chambers has been employed to allow chronic observation of the microcirculation in intact, non-anaesthetized animals. This paper reports results with two transparent chamber models: the skin-fold chamber model in the hamster and the mouse. An overview is provided of the technical development of the chamber technique and of the various experimental studies that have been performed using these models in ischaemia-reperfusion of striated muscle. Particular emphasis is given to a description of the methods used to induce ischaemia and reperfusion, namely pressure-induced or tourniquet-induced ischaemia. While the former allows simulation of the pathophysiological situation in compartment syndromes, the latter provides an appropriate simulation of the clinical situation in vascular, transplantation and reconstructive surgery. Future possibilities for these microcirculation models in research into the pathophysiology of ischemia-reperfusion are outlined, and potential therapeutic measures to preserve postischaemic tissue are discussed.