Bacillus thuringiensis Cry11Ba works synergistically with Cry4Aa but not with Cry11Aa for toxicity against mosquito Culex pipiens (Diptera: Culicidae) larvae.
Bacillus thuringiensis Cry11Ba works synergistically with Cry4Aa but not with Cry11Aa for toxicity against mosquito Culex pipiens (Diptera: Culicidae) larvae.
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苏云金芽孢杆菌 Cry11Ba 与 Cry4Aa 具有协同作用，但与 Cry11Aa 没有协同作用，对淡色库蚊（双翅目：库蚊科）幼虫具有毒性。
DOI：
10.1007/s13355-016-0454-z
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发表时间：
2017
影响因子：
1.3
通讯作者：
Ide T
中科院分区：
文献类型：
--
作者：
Hayakawa T;Yoneda N;Okada K;Higaki A;Howlader MTH;Ide T
设计了编码苏云金芽孢杆菌jegathesan亚种Cry11Ba的2,175 bp修饰基因(cry11Ba-S1)，并在大肠杆菌中将重组蛋白表达为与谷胱甘肽S-转移酶的融合蛋白。重组Cry11Ba对淡色库蚊幼虫具有高毒性，其毒性分别是苏云金芽孢杆菌以色列亚种杀蚊剂Cry4Aa和Cry11Aa的9倍和17倍。有趣的是，通过与 Cry4Aa 混合，重组 Cry11Ba 的毒性进一步增加，但与 Cry11Aa 混合则不然。这些发现表明，Cry11Ba 与 Cry4Aa 具有协同作用，但与 Cry11Aa 没有协同作用，表现出对 Cry4Aa 的毒性。 pipienslarvae。另一方面，与刷状缘膜囊泡 (BBMV) 结合的 Cry 毒素的量在单个毒素和毒素混合物之间没有显着变化，这表明与 BBMV 结合的毒素的增加并不是观察到协同效应的原因。人们普遍认为，毒素的协同作用是增强杀虫活性和控制蚊子 Cry 毒素抗性的潜在强大工具。 Cry4Aa 和 Cry11Ba 的混合物可增加毒性，这对于控制蚊子非常有价值。
A 2,175-bp modified gene (cry11Ba-S1) encoding Cry11Ba fromBacillus thuringiensissubsp.jegathesanwas designed and the recombinant protein was expressed as a fusion protein with glutathioneS-transferase inEscherichia coli. The recombinant Cry11Ba was highly toxic againstCulex pipiensmosquito larvae, being nine and 17 times more toxic than mosquitocidal Cry4Aa and Cry11Aa fromBacillus thuringiensissubsp.israelensis, respectively. Interestingly, a further increase in the toxicity of the recombinant Cry11Ba was achieved by mixing with Cry4Aa, but not with Cry11Aa. These findings suggested that Cry11Ba worked synergistically with Cry4Aa, but not with Cry11Aa, in exhibiting toxicity againstC. pipienslarvae. On the other hand, the amount of Cry toxin bound to brush border membrane vesicles (BBMVs) did not significantly change between individual toxins and the toxin mixtures, suggesting that the increase in toxins binding to BBMVs was not a reason for the observed synergistic effect. It is generally accepted that synergism of toxins is a potentially powerful tool for enhancing insecticidal activity and managing Cry toxin resistance in mosquitoes. The mixture of Cry4Aa and Cry11Ba in order to increase toxicity would be very valuable in terms of mosquito control.