Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo.
Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo.
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DOI：
10.1158/2159-8290.cd-12-0503
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发表时间：
2013-06
期刊：
影响因子：
28.2
通讯作者：
Piwnica-Worms D
中科院分区：
文献类型：
--
作者：
Kocher B;Piwnica-Worms D
生物发光成像（BLI）是一种强大的非侵入性工具，在过去十年中极大地加速了癌症系统的体内研究以及癌症小鼠模型的纵向分析。多种荧光素酶已通过基因工程导入癌症小鼠模型（GEMMs）中，这使得能够研究与活细胞和动物体内的致癌转录、转录后加工、蛋白质 - 蛋白质相互作用、转化以及癌基因成瘾相关的细胞和分子事件。与荧光素酶偶联的GEMMs最终成为一种非侵入性、可重复、纵向且符合生理的手段，通过该手段可以在活体动物的原位环境中准确研究癌症系统和治疗反应。
Bioluminescent imaging (BLI) is a powerful non-invasive tool that has dramatically accelerated the in vivo interrogation of cancer systems and longitudinal analysis of mouse models of cancer over the past decade. Various luciferase enzymes have been genetically engineered into mouse models (GEMMs) of cancer which permit investigation of cellular and molecular events associated with oncogenic transcription, post-transcriptional processing, protein-protein interactions, transformation and oncogene addiction in live cells and animals. Luciferase-coupled GEMMs ultimately serve as a non-invasive, repetitive, longitudinal, and physiological means by which cancer systems and therapeutic responses can be investigated accurately within the autochthonous context of a living animal.