Expression of transforming growth factor-beta isoforms (beta 2 and beta 3) in the mouse uterus: analysis of the periimplantation period and effects of ovarian steroids.
Expression of transforming growth factor-beta isoforms (beta 2 and beta 3) in the mouse uterus: analysis of the periimplantation period and effects of ovarian steroids.
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DOI：
10.1210/endo.130.6.1375903
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发表时间：
1992-06
期刊：
影响因子：
4.8
通讯作者：
S. Das;K. Flanders;G. Andrews;S. Dey
中科院分区：
文献类型：
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作者：
S. Das;K. Flanders;G. Andrews;S. Dey
使用 Northern 印迹杂交和/或免疫细胞化学研究了小鼠子宫在植入期间以及对 17β-雌二醇 (E2) 和孕酮 (P4) 急性暴露的反应中 β 型转化生长因子基因（TGF β 2 和 TGF β 3）的表达。采用 TGF β 2 或 TGF β 3 特异性的多克隆抗肽抗体进行免疫细胞化学。在植入前的子宫中[怀孕第 1-4 天 (D)；第1天=阴道塞]，在管腔和腺上皮以及子宫肌层和血管平滑肌中观察到TGFβ2的免疫染色。在着床后时期（D5-D8），还在蜕膜细胞中检测到TGFβ2免疫染色。相反，在整个植入期间（D1-D8），TGFβ3 免疫染色仅限于子宫肌层和血管平滑肌。反义 TGF β 2 和 TGF β 3 RNA 探针用于 Northern 印迹。 Northern 印迹杂交显示 D1-D6 上的总子宫 Poly(A)+ RNA 以及妊娠 D7 和 D8 收集的蜕膜和子宫肌层的 Poly(A)+ RNA 中有 4 个 TGF beta 2 转录物（大约 6.0、5.0、4.0 和 3.5 kilobase）。在从 D8 假孕小鼠获得的蜕膜细胞或子宫肌层的分离样品中也检测到了这些 TGF β 2 转录物，其中在 D4 上通过实验诱导了蜕膜细胞反应。这些转录物的水平在植入期间保持相对恒定。 Northern 印迹分析在 D1-D6 的总子宫 Poly(A)+ RNA 中检测到 3.8 KB 的 TGF beta 3 转录物。在妊娠 D7 和 D8 或假妊娠 D8 的子宫肌层 RNA 样本中检测到该转录本，但在 D7 和 D8 的蜕膜 RNA 或 D8 的蜕膜 RNA 中未检测到该转录本。在成年卵巢切除小鼠的子宫中检查了卵巢类固醇对 TGF β 2 和 TGF β 3 mRNA 的影响。子宫 TGF β 2 或 TGF β 3 mRNA 在卵巢切除小鼠中持续存在。然而，注射 E2 会诱导快速（6 小时）但短暂的 TGF β 2 mRNA 诱导（约 3 至 4 倍）。注射 P4 对 TGF β 2 mRNA 水平没有影响，P4 与 E2 共注射不会拮抗 E2 刺激的 TGF β 2 mRNA 瞬时积累。相比之下，注射 E2 或注射 P4 均未对 TGF beta 3 mRNA 水平产生显着影响。（摘要截断为 400 字）
Expression of beta-type transforming growth factor genes (TGF beta 2 and TGF beta 3) in the mouse uterus during the periimplantation period and in response to an acute exposure to 17 beta-estradiol (E2) and progesterone (P4) was studied using Northern blot hybridization and/or immunocytochemistry. Polyclonal antipeptide antibodies specific for TGF beta 2 or TGF beta 3 were employed for immunocytochemistry. In the preimplantation uterus [days (D) 1-4 of pregnancy; day 1 = vaginal plug], immunostaining for TGF beta 2 was observed in luminal and glandular epithelia as well as in myometrium and vascular smooth muscle. In the postimplantation period (D5-D8), TGF beta 2 immunostaining was also detected in decidual cells. In contrast, TGF beta 3 immunostaining was restricted to the myometrium and vascular smooth muscle throughout the periimplantation period (D1-D8). Antisense TGF beta 2 and TGF beta 3 RNA probes were employed for Northern blotting. Northern blot hybridization revealed four TGF beta 2 transcripts (approximately 6.0, 5.0, 4.0, and 3.5 kilobases) in total uterine poly(A)+ RNA on D1-D6 and in poly(A)+ RNA from the deciduum and myometrium collected on D7 and D8 of pregnancy. These TGF beta 2 transcripts were also detected in isolated samples of deciduomata or myometrium obtained from D8 pseudopregnant mice in which the decidual cell reaction was induced experimentally on D4. The levels of these transcripts remained relatively constant during the periimplantation period. Northern blot analysis detected a 3.8-kilobase TGF beta 3 transcript in total uterine poly(A)+ RNA on D1-D6. This transcript was detected in myometrial RNA samples on D7 and D8 of pregnancy or D8 of pseudopregnancy, but was not detected in RNA from the deciduum on D7 and D8 or in that from deciduomata on D8. The effects of ovarian steroids on TGF beta 2 and TGF beta 3 mRNAs were examined in uteri of adult ovariectomized mice. Uterine TGF beta 2 or TGF beta 3 mRNA persisted in ovariectomized mice. However, an injection of E2 induced a rapid (6 h), but transient, induction (approximately 3- to 4-fold) of TGF beta 2 mRNA. An injection of P4 had no effect on TGF beta 2 mRNA levels, and coinjection of P4 with E2 did not antagonize the E2-stimulated transient accumulation of TGF beta 2 mRNA. In comparison, neither an injection of E2 nor one of P4 exerted significant effects on TGF beta 3 mRNA levels.(ABSTRACT TRUNCATED AT 400 WORDS)